Analysis of Mutant Manganese-Superoxide Dismutase on the Lifespan of Saccharomyces cerevisiae""

Previously isolated mutants of the superoxide dismutase 2 gene (SOD2), selected
for increased activity in E. coli, were transformed into yeast cells in order to observe their
effects on aging. Polymerase chain reaction and DNA sequencing were used to confirm
that the yeast incorporated the mutant form of the gene. The strains with mutant forms of
the SOD2 gene were observed to have decreased growth rates compared to the unmutated
strain. Lifespan analyses were then conducted in order to see if yeast with
mutant versions of SOD2 had lifespans that differed significantly from those with the unmutated
forms of the gene. The mutant forms of SOD2 had no significant effect on the
lifespan of yeast.