Assessment of multiple paternity for the queen conch, Strombus gigas

The commercially important queen conch, Strombus gigas, has been observed copulating with multiple partners and laying multiple egg masses during a reproductive season (Randall, 1964). While multiple paternity has been confirmed using microsatellite based genetic analysis for a variety of other gastropods, this technique has not been employed for S. gigas. Determining whether or not this species is capable of multiple paternity is important to understanding and maintaining genetic diversity of natural and captive populations. While an assessment of multiple paternity is the ultimate goal of this study, for my thesis, I have completed preliminary work which includes perfecting methods of tissue collection, DNA extraction, and DNA amplification with six non-labeled polymorphic microsatellite molecular markers, using cultured Strombus gigas animals. In addition, I collected tissue and extracted DNA from three wild S. gigas adult females and their egg masses from Pelican Shoal in the Florida Keys.