Bacillus strains with >99.7% 16S rRNA gene sequence similarity were characterized with DNA:DNA hybridization, cellular fatty acid (CFA) analysis, and testing of 100 phenotypic traits.When paired with the most closely related type strain, percent DNA:DNA similarities (% S) for six Bacillus strains were all far below the recommended 70% threshold value for species circumscription with Bacillus nealsonii.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This publication is available at http://www.hindawi.com/journals/ijmb/2011/673136 and may be cited as: Peak, K. K., Duncan, K. E., Luna, V. A., King, D. S., McCarthy, P. J., & Cannons, A. C. (2011). Bacillus strains most closely-related to Bacillus nealsonii are not effectively circumscribed within the taxonomic species definition. International Journal of Microbiology, 2011, Article ID 673136, 1-13. doi:10.1155/2011/673136
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1838.