Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2007
EDTF Date Created
2007
Description
The RRE is a sequence of the HIV genome which is required for the export of the
unspliced mRNA from the nucleus to the cytoplasm. Previous studies show that RRE
on the HIV mRNA binds directly to Rev which then interacts with Ran and CRM 1 to
form an export complex. Our results indicate that Ran can interact with the RRE in
the absence of Rev and CRM 1 but in the presence of other factor(s) present in the nuclear extract. Ran-GEF or RCC 1 seems to be a potential mediating factor. Our
results suggest that Ran binds directly to RCC 1 and that the binding is disrupted by
addition of excess nucleotides and magnesimn. Our suggestion is that Ran and RCC 1
are members of an alternate export pathway present in the HIV. Our observation that the binding is nonspecific makes us speculate that this export pathway may be present
in other cell types as well.
unspliced mRNA from the nucleus to the cytoplasm. Previous studies show that RRE
on the HIV mRNA binds directly to Rev which then interacts with Ran and CRM 1 to
form an export complex. Our results indicate that Ran can interact with the RRE in
the absence of Rev and CRM 1 but in the presence of other factor(s) present in the nuclear extract. Ran-GEF or RCC 1 seems to be a potential mediating factor. Our
results suggest that Ran binds directly to RCC 1 and that the binding is disrupted by
addition of excess nucleotides and magnesimn. Our suggestion is that Ran and RCC 1
are members of an alternate export pathway present in the HIV. Our observation that the binding is nonspecific makes us speculate that this export pathway may be present
in other cell types as well.
Language
Type
Form
Extent
85 p.
Subject (Topical)
Identifier
FA00000742
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2007.
Charles E. Schmidt College of Medicine
FAU Electronic Theses and Dissertations Collection
Date Backup
2007
Date Created Backup
2007
Date Text
2007
Date Created (EDTF)
2007
Date Issued (EDTF)
2007
Extension
FAU
IID
FA00000742
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
Dhir, Neetika
Graduate College
Physical Description
application/pdf
85 p.
Title Plain
Characterization of Cellular Proteins Binding HIV's Rev Responsive Element (RRE)
Use and Reproduction
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Origin Information
2007
2007
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Characterization of Cellular Proteins Binding HIV's Rev Responsive Element (RRE)
Other Title Info
Characterization of Cellular Proteins Binding HIV's Rev Responsive Element (RRE)