Characterization of genetic markers for in vitro cell line identification of the marine sponge, Axinella corrugata

Member of
HBOI Faculty Contributions
Contributors
Lopez, Jose V.
Peterson, C. L.
Willoughby, Robin
Wright, Amy E.
Enright, E.
Zoladz, S.
Pomponi, Shirley A.
Publisher
Oxford University Press
Date Issued
2002
Note

The marine sponge Axinella corrugata is being developed as a model organism for in vitro marine invertebrate research. Molecular genetics methods such as DNA fingerprinting [amplified fragment length polymorphism (AFLP) and single-stranded conformation polymorphism (SSCP)] and single-locus DNA sequence analyses were applied to this model to meet the primary objective of identifying positive A. corrugata–specific molecular markers that will aid in verifying cell identity in vitro and distinguish sponge cells from potential microbial contaminants.

Language
English
Type
Text
Genre
article
Extent
11 p.
Subject (Topical)
Sponges--Research
Marine invertebrates
Genetic markers
Identifier
3355156
Additional Information
The marine sponge Axinella corrugata is being developed as a model organism for in vitro marine invertebrate research. Molecular genetics methods such as DNA fingerprinting [amplified fragment length polymorphism (AFLP) and single-stranded conformation polymorphism (SSCP)] and single-locus DNA sequence analyses were applied to this model to meet the primary objective of identifying positive A. corrugata–specific molecular markers that will aid in verifying cell identity in vitro and distinguish sponge cells from potential microbial contaminants.
This is an electronic version of an article published in Journal of Heredity, http://jhered.oxfordjournals.org/, and may be cited as: Lopez, J. V., Peterson, C. L., Willoughby, R., Wright, A. E., Enright, E., Zoladz, S., & Pomponi, S. A. (2002). Characterization of genetic markers for in vitro cell line identification of the marine sponge, Axinella corrugata. Journal of Heredity, 93(1), 27-36. doi:10.1093/jhered/93.1.27
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1451.
Date Backup
2002
Date Text
2002
DOI
10.1093/jhered/93.1.27
Date Issued (EDTF)
2002
Extension


FAU
FAU
admin_unit="FAU01", ingest_id="ing14254", creator="creator:BCHANG", creation_date="2012-11-29 10:25:18", modified_by="super:FAUDIG", modification_date="2014-02-07 16:29:30"

IID
FADT3355156
Issuance
single unit
Person Preferred Name

Lopez, Jose V.

creator

Physical Description

pdf
11 p.
PURL
http://purl.flvc.org/FCLA/DT/3355156
Title Plain
Characterization of genetic markers for in vitro cell line identification of the marine sponge, Axinella corrugata
URL
http://purl.flvc.org/FCLA/DT/3355156
Origin Information

Oxford University Press
2002
single unit
Title
Characterization of genetic markers for in vitro cell line identification of the marine sponge, Axinella corrugata
Other Title Info

Characterization of genetic markers for in vitro cell line identification of the marine sponge, Axinella corrugata