Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2006
EDTF Date Created
2006
Description
One methionine sulfoxide reductase A (TMSRA) and two methionine sulfoxide reductase
Bs (TMSRB 1 and TMSRB2) were isolated from tobacco plants. TMSRA showed
specificity for the reduction of Met-(S)-SO and both TMSRBs were specific for the
reduction of Met-(R)-SO. TMSRA was the cytosolic form and both TMSRBs were
plastid forms based on sequence comparison and expression tests. TMSRA and TMSRB2
could use either thioredoxin (TRX) or dithiothreitol (DTT) as reducing system, while
TMSRB 1 showed little activity with TRX but much more activity with DTT, which was
similar to the mitochondrial MSRB2 from mammals. Ferredoxin (FD) is not the reducing
system for Msrs, but might reflect the redox status in the cell and control the activity of
Msrs indirectly.
Bs (TMSRB 1 and TMSRB2) were isolated from tobacco plants. TMSRA showed
specificity for the reduction of Met-(S)-SO and both TMSRBs were specific for the
reduction of Met-(R)-SO. TMSRA was the cytosolic form and both TMSRBs were
plastid forms based on sequence comparison and expression tests. TMSRA and TMSRB2
could use either thioredoxin (TRX) or dithiothreitol (DTT) as reducing system, while
TMSRB 1 showed little activity with TRX but much more activity with DTT, which was
similar to the mitochondrial MSRB2 from mammals. Ferredoxin (FD) is not the reducing
system for Msrs, but might reflect the redox status in the cell and control the activity of
Msrs indirectly.
Language
Type
Form
Extent
55 p.
Subject (Topical)
Identifier
FA00000746
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2006.
Charles E. Schmidt College of Science
FAU Electronic Theses and Dissertations Collection
Date Backup
2006
Date Created Backup
2006
Date Text
2006
Date Created (EDTF)
2006
Date Issued (EDTF)
2006
Extension
FAU
IID
FA00000746
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
Ding, Di
Graduate College
Physical Description
application/pdf
55 p.
Title Plain
Characterization of Methionine Sulfoxide Reductases A and Bs from Tobacco Plant
Use and Reproduction
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Origin Information
2006
2006
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Characterization of Methionine Sulfoxide Reductases A and Bs from Tobacco Plant
Other Title Info
Characterization of Methionine Sulfoxide Reductases A and Bs from Tobacco Plant