Characterization of a novel close-to-root papillomavirus from aFlorida manatee by using multiply primed rolling-circle amplification: Trichechus manatus latirostrispapillomavirus type 1

By using an isothermal multiply primed rolling-circle amplification protocol, the complete genomic DNA of
a novel papillomavirus was amplified from a skin lesion biopsy of a Florida manatee (Trichechus manatus
latirostris), one of the most endangered marine mammals in United States coastal waters. The nucleotide
sequence, genome organization, and phylogenetic position of the Trichechus manatus latirostris papillomavirus
type 1 (TmPV-1) were determined. TmPV-1 is the first virus isolated from the order of Sirenia. A phylogenetic
analysis shows that TmPV-1 is only distantly related to other papillomavirus sequences, and it appears in our phylogenetic tree as a novel close-to-root papillomavirus genus.