Note
Thesis (M.S.)--Florida Atlantic University, 1972.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
1972
Description
The effects of added salt on the deacylation rate constant of
trans-cinnamoylchymotrypsin were examined. Ionic strength effects on
the equilibrium constant of an ionizable, enzymic group of pKa 7.25
were also studied. Through the development of methods for the precise
measurement of pH and observed rate constants, very small changes
in the experimental parameters could be detected. It was found that
neither the deacylation rate constant nor the kinetically determined pKa exhibited a significant salt effect over the ionic strength range of 0.04
to 0.12 M. Based on the known three-dimensional structure of the
active site of chymotrypsin, four plausible mechanisms were considered
in light of the experimental absence of salt effects. The presence of calcium ion at low concentrations significantly perturbed
the values of the deacylation rate constant and the protonic equilibrium
constant.
trans-cinnamoylchymotrypsin were examined. Ionic strength effects on
the equilibrium constant of an ionizable, enzymic group of pKa 7.25
were also studied. Through the development of methods for the precise
measurement of pH and observed rate constants, very small changes
in the experimental parameters could be detected. It was found that
neither the deacylation rate constant nor the kinetically determined pKa exhibited a significant salt effect over the ionic strength range of 0.04
to 0.12 M. Based on the known three-dimensional structure of the
active site of chymotrypsin, four plausible mechanisms were considered
in light of the experimental absence of salt effects. The presence of calcium ion at low concentrations significantly perturbed
the values of the deacylation rate constant and the protonic equilibrium
constant.
Language
Type
Form
Extent
83 p.
Subject (Topical)
Identifier
13496
Additional Information
Thesis (M.S.)--Florida Atlantic University, 1972.
Charles E. Schmidt College of Science
FAU Electronic Theses and Dissertations Collection
Date Backup
1972
Date Text
1972
Date Issued (EDTF)
1972
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-19 01:10:32", modified_by="staff:fcllz", modification_date="2011-01-06 13:08:49"
IID
FADT13496
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
COLEMAN, DANIEL R.
Graduate College
Physical Description
83 p.
application/pdf
Title Plain
CHARGE DISTRIBUTION IN CHYMOTRYPSIN CATALYSIS
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
1972
monographic
Boca Raton, Fla.
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
CHARGE DISTRIBUTION IN CHYMOTRYPSIN CATALYSIS
Other Title Info
CHARGE DISTRIBUTION IN CHYMOTRYPSIN CATALYSIS