The purpose of this study was to detect if gender affected the diagnostic and therapeutic procedures for chest pain in an Emergency Department (ED). This study evaluated the use of a chest pain assessment, Electrocardiograph (ECG), care path protocol, myocardial markers and notification of a cardiologist for an acute myocardial infarction (AMI), as well as, elapsed time of ED arrival to 1st ECG, and 1st ECG interpretation. The eleven-month retrospective analyses used abstracted data on 1870 discharges. Chi-squared analysis and ANOVA were used to determine if a gender bias existed in the use of the different diagnostic procedures (p < 0.05). Results indicated men were not treated more aggressively on the initial presentation of chest pain in the ED. Our findings may suggest that the use of a care path protocol by well-trained ED physicians at this hospital helped in identifying the typical and atypical presentations of chest pain regardless of gender.