Note
FAU Electronic Theses and Dissertations Collection
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2003
Description
This study developed a method for positive selection of anti-single stranded (ss)DNA B cells from peripheral blood mononuclear cells (PBMCs) using a twenty nucleotide oligomer of deoxythymidine (oligo(dT) 20mer), coupled to microscopie magnetic beads. Oligo(dT) 20mer specificity for plasma anti-ssDNA antibody (Ab) was established through indirect enzyme linked immunosorbent assay (ELISA) and flow cytometry. A novel method for semi-quantitative detection of ssDNA specific, B cells from PBMC purified peripheral blood that utilized flow cytometric analysis of oligo(dT) 20mer Texas Red labeled cells was developed. Qualitative purification of ssDNA specific, B cells using oligo(dT) coupled magnetic beads was determined through light microscopy and flow cytometric analysis of positive sclected cell populations. Cross-reaetivity of oligo(dT) 20mer with receptors distinct from membrane Ab, resulted in the use of oligo(dC) 20mer as a useful blocking agent. Results show anti-ssDNA Ab titer does not correlate with numbers of peripheral blood ssDNA specific, B cells.
Language
Type
Form
Extent
76 p.
Subject (Topical)
Identifier
9780496219247
ISBN
9780496219247
Additional Information
FAU Electronic Theses and Dissertations Collection
Thesis (M.S.)--Florida Atlantic University, 2003.
Charles E. Schmidt College of Science
Date Backup
2003
Date Text
2003
Date Issued (EDTF)
2003
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-18 22:21:12", modified_by="staff:fcllz", modification_date="2011-01-06 13:08:53"
IID
FADT13086
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
Saccocio, Seth.
Graduate College
Physical Description
76 p.
application/pdf
Title Plain
Detection and purification of lupus B cells
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
2003
monographic
Boca Raton, Fla.
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Detection and purification of lupus B cells
Other Title Info
Detection and purification of lupus B cells