Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2014
EDTF Date Created
2014
Description
This study evaluated the stability of oral bacteria in healthy subjects and documented
community shifts in smokers and oral/periodontal disease by employing PCR-RFLP,
DGGE and sequence analysis of the 16S rDNA gene from metagenomes and plate-wash
(cultured) bacteria of oral wash from 15 participants,. A stable core of bacterial DNA
fingerprint was detected within and between subjects and did not change over time when
analyzed in smokers and healthy non-smokers. Signature bands in smokers, non-smokers
and periodontal disease subjects were evident suggesting the presence of potential
indicators of health and poor oral health. Taxon diversity was higher in smokers
including members of the genera Rothia, Synechococcus, Neisseria, Thiomargarita and
Pyrobaculum but highest in periodontal disease. The two techniques successfully aligned
the subjects within appropriate categories (based on their oral microbial genetic
patterns)confirming their diagnostic suitability.
community shifts in smokers and oral/periodontal disease by employing PCR-RFLP,
DGGE and sequence analysis of the 16S rDNA gene from metagenomes and plate-wash
(cultured) bacteria of oral wash from 15 participants,. A stable core of bacterial DNA
fingerprint was detected within and between subjects and did not change over time when
analyzed in smokers and healthy non-smokers. Signature bands in smokers, non-smokers
and periodontal disease subjects were evident suggesting the presence of potential
indicators of health and poor oral health. Taxon diversity was higher in smokers
including members of the genera Rothia, Synechococcus, Neisseria, Thiomargarita and
Pyrobaculum but highest in periodontal disease. The two techniques successfully aligned
the subjects within appropriate categories (based on their oral microbial genetic
patterns)confirming their diagnostic suitability.
Language
Type
Form
Extent
105 p.
Subject (Topical)
Identifier
FA00004184
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2014.
FAU Electronic Theses and Dissertations Collection
Date Backup
2014
Date Created Backup
2014
Date Text
2014
Date Created (EDTF)
2014
Date Issued (EDTF)
2014
Extension
FAU
IID
FA00004184
Organizations
Attributed name: Florida Atlantic University
Attributed name: Charles E. Schmidt College of Science
Attributed name: Department of Biological Sciences
Person Preferred Name
Chakraborty, Shreyasee
author
Graduate College
Physical Description
application/pdf
105 p.
Title Plain
DNA fingerprints of human oral microbiome: a first step towards early diagnosis of oral diseases
Use and Reproduction
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
2014
2014
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
DNA fingerprints of human oral microbiome: a first step towards early diagnosis of oral diseases
Other Title Info
DNA fingerprints of human oral microbiome: a first step towards early diagnosis of oral diseases