effect of mutated aconitase on yeast longevity

Member of
Honors Student Theses
Contributors
Kwan, CJ.
Publisher
Florida Atlantic University
Date Issued
2012
Description
Aconitase is an important enzyme in the citric Acid Cycle, is needed for maintenance of mitochondrial DNA, is a key regulator of iron in the cell, and is very sensitive to oxidative stress. We have isolatd the yeast ACO1 gene, which codes for aconitase, and randomly mutated it to create a mutant library of cells each expressing a different version of ACO1. We will select for oxidative stress resistant aconitase in S. cerevisiae by subjecting strains to successive rounds of heat shock and competitive growth against other mutants. The "winner" of this competition will then be analyzed for which version of aconitase it is expressing, which may lead to increased longevity.
Note

by CJ Kwan.

Language
English
Type
Text
Genre
thesis
Form
electronic
electronic resource
Extent
vi, 25 p. : ill.
Subject (Topical)
Yeast fungi
Research
Microbial genetics
Aging
Molecular aspects
Mutation (Biology)
Cell metabolism
Identifier
818783324
OCLC Number
818783324
Rights

Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.

Additional Information
by CJ Kwan.
Thesis (B.A.)--Florida Atlantic University, 2012.
Includes bibliography.
Electronic reproduction. Boca Raton, Fla., 2012. Mode of access: World Wide Web.
Date Backup
2012
Date Text
2012
Date Issued (EDTF)
2012
Extension


FAU
FAU
admin_unit="FAU01", ingest_id="ing15015", creator="creator:NBURWICK", creation_date="2013-04-12 15:15:31", modified_by="super:FAUDIG", modification_date="2013-04-19 09:44:16"

IID
FADT3359310
Issuance
multipart monograph
Organizations
Attributed name: Harriet L. Wilkes Honors College
Person Preferred Name

Kwan, CJ.
Physical Description

electronic
electronic resource
vi, 25 p. : ill.
PURL
http://purl.flvc.org/FAU/3359310
Title Plain
effect of mutated aconitase on yeast longevity
URL
http://purl.flvc.org/FAU/3359310
Use and Reproduction
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Origin Information

2012
multipart monograph
Florida Atlantic University
Physical Location
FBoU FAUER
Title
effect of mutated aconitase on yeast longevity
Other Title Info

The
effect of mutated aconitase on yeast longevity