Note
Thesis (M.S.)--Florida Atlantic University, 1985.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
1985
Description
The objective of this study was to elucidate the
interaction of the carcinogen MNNG with nuclear DNA of Rat
Hepatoma cells. The effect of a range of MNNG concentrations
on RH cell DNA was studied by an analysis of the DNA
fragments obtained in linear alkaline sucrose gradients. A macromolecular analysis of the sedimentation profile for
0.06 mM MNNG (Experiment I), and 0.1 mM MNNG (Experiment
III), suggested that the system was paucidisperse and
contained at least three components. The major component
Max 1 has a molecular weight comparable to that known for
the mammalian replicon. Max 2 has a molecular weight
twice that of Max 1 and Max 3 has a molecular weight half
that of Max 1. The size of the replicon is comparable to
that obtained by others. Inferences were drawn regarding
the structure of chromatin and the role of the distribution
of sites hypersensitive to methylation with respect
to the oncogenes.
interaction of the carcinogen MNNG with nuclear DNA of Rat
Hepatoma cells. The effect of a range of MNNG concentrations
on RH cell DNA was studied by an analysis of the DNA
fragments obtained in linear alkaline sucrose gradients. A macromolecular analysis of the sedimentation profile for
0.06 mM MNNG (Experiment I), and 0.1 mM MNNG (Experiment
III), suggested that the system was paucidisperse and
contained at least three components. The major component
Max 1 has a molecular weight comparable to that known for
the mammalian replicon. Max 2 has a molecular weight
twice that of Max 1 and Max 3 has a molecular weight half
that of Max 1. The size of the replicon is comparable to
that obtained by others. Inferences were drawn regarding
the structure of chromatin and the role of the distribution
of sites hypersensitive to methylation with respect
to the oncogenes.
Language
Type
Form
Extent
213 p.
Subject (Topical)
Identifier
14260
Additional Information
Thesis (M.S.)--Florida Atlantic University, 1985.
Charles E. Schmidt College of Science
FAU Electronic Theses and Dissertations Collection
Date Backup
1985
Date Text
1985
Date Issued (EDTF)
1985
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-19 02:20:55", modified_by="staff:fcllz", modification_date="2011-01-06 13:09:07"
IID
FADT14260
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Attributed name: Charles E. Schmidt College of Science
Attributed name: Department of Biological Sciences
Person Preferred Name
JHABVALA, PERSEUS
Graduate College
Physical Description
213 p.
application/pdf
Title Plain
EFFECT OF N-METHYL-N'-NITRO-N-NITROSOGUANIDINE (MNNG) ON DNA OF CULTURED RAT HEPATOMA CELLS
Use and Reproduction
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Origin Information
1985
monographic
Boca Raton, FL
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, FL
Sub Location
Digital Library
Title
EFFECT OF N-METHYL-N'-NITRO-N-NITROSOGUANIDINE (MNNG) ON DNA OF CULTURED RAT HEPATOMA CELLS
Other Title Info
EFFECT OF N-METHYL-N'-NITRO-N-NITROSOGUANIDINE (MNNG) ON DNA OF CULTURED RAT HEPATOMA CELLS