Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2018
EDTF Date Created
2018
Description
This project was designed to investigate the effects of carbon dioxide (CO2) levels on the
growth and pigment ratios (chemotaxonomy) of freshwater algal species typical to the
south Florida surface waters. Green algae, diatoms, and cyanobacteria were cultured
under 400 or 800 ppm CO3 in air for several weeks. Growth monitoring used a cell
counter, hemocytometer, and chlorophyll fluorescence. Pigments were analyzed using
HPLC-PDA. Experiments with certified CO2 concentrations (400, 600, 800, 1200 ppm)
were conducted with helium degassed ultrapure water and each of three culture media.
Theoretical and experimental pH values with water matched exactly. However, each
culture media proved to exhibit significant buffer capacity. Cell growth monitoring was
problematic except for the cyanobacterium Microcystis aeruginosa. That species
responded to increased CO2 (800 ppm) with increased growth rates as predicted. The
other species gave erratic results mainly due to difficulties in obtaining valid consistent
cell counts.
growth and pigment ratios (chemotaxonomy) of freshwater algal species typical to the
south Florida surface waters. Green algae, diatoms, and cyanobacteria were cultured
under 400 or 800 ppm CO3 in air for several weeks. Growth monitoring used a cell
counter, hemocytometer, and chlorophyll fluorescence. Pigments were analyzed using
HPLC-PDA. Experiments with certified CO2 concentrations (400, 600, 800, 1200 ppm)
were conducted with helium degassed ultrapure water and each of three culture media.
Theoretical and experimental pH values with water matched exactly. However, each
culture media proved to exhibit significant buffer capacity. Cell growth monitoring was
problematic except for the cyanobacterium Microcystis aeruginosa. That species
responded to increased CO2 (800 ppm) with increased growth rates as predicted. The
other species gave erratic results mainly due to difficulties in obtaining valid consistent
cell counts.
Language
Type
Form
Extent
83 p.
Subject (Topical)
Identifier
FA00013170
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2018.
FAU Electronic Theses and Dissertations Collection
Date Backup
2018
Date Created Backup
2018
Date Text
2018
Date Created (EDTF)
2018
Date Issued (EDTF)
2018
Extension
FAU
IID
FA00013170
Organizations
Attributed name: Florida Atlantic University
Attributed name: Charles E. Schmidt College of Science
Attributed name: Department of Chemistry and Biochemistry
Person Preferred Name
Bermudez, Jeanne
author
Graduate College
Physical Description
application/pdf
83 p.
Title Plain
Effects of Carbon Dioxide Levels on Growth and Pigments of Freshwater Algae
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
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Origin Information
2018
2018
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Effects of Carbon Dioxide Levels on Growth and Pigments of Freshwater Algae
Other Title Info
Effects of Carbon Dioxide Levels on Growth and Pigments of Freshwater Algae