Note
Thesis (M.S.)--Florida Atlantic University, 1973.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
1973
Description
Leukocytes, harvested from EIA infected horse plasma, were
disrupted and partially purified by differential centrifugation.
This material was assayed for its capacity to inhibit migration
of leukocytes from infected horses. After 6 X 10^6 r of gamma
radiation the material was reassayed and 85% of the initial
activity remained, indicating that little antigenic destruction
had occurred. This material was coupled to rabbit gamma
globulin and used for horse inoculation. The above material,
uncoupled, and a partially purified irradiated normal horse
protein material were used as controls. Responses to
immunizations and challenges were monitored. Uncoupled irradiated
material produced the disease. Coupled irradiated
material did not produce the disease and the immunized horse
was resistant to challenge with 1 ml of a 10^-3
dilution of infectious serum for 30 days and, then, to 1 ml of undiluted
infectious serum for 30 more days .
disrupted and partially purified by differential centrifugation.
This material was assayed for its capacity to inhibit migration
of leukocytes from infected horses. After 6 X 10^6 r of gamma
radiation the material was reassayed and 85% of the initial
activity remained, indicating that little antigenic destruction
had occurred. This material was coupled to rabbit gamma
globulin and used for horse inoculation. The above material,
uncoupled, and a partially purified irradiated normal horse
protein material were used as controls. Responses to
immunizations and challenges were monitored. Uncoupled irradiated
material produced the disease. Coupled irradiated
material did not produce the disease and the immunized horse
was resistant to challenge with 1 ml of a 10^-3
dilution of infectious serum for 30 days and, then, to 1 ml of undiluted
infectious serum for 30 more days .
Language
Type
Form
Extent
86 p.
Subject (Topical)
Identifier
13566
Additional Information
Thesis (M.S.)--Florida Atlantic University, 1973.
Charles E. Schmidt College of Science
FAU Electronic Theses and Dissertations Collection
Date Backup
1973
Date Text
1973
Date Issued (EDTF)
1973
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-19 01:16:51", modified_by="staff:fcllz", modification_date="2011-01-06 13:08:57"
IID
FADT13566
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
BELTRANENA, HUMBERTO PEDRO PABLO.
Graduate College
Physical Description
86 p.
application/pdf
Title Plain
EVALUATION OF A GAMMA-IRRADIATED, GAMMA GLOBULIN COUPLED, PARTIALLY PURIFIED MATERIAL AS AN IMMUNIZING AGENT FOR EQUINE INFECTIOUS ANEMIA (EIA)
Use and Reproduction
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
1973
monographic
Boca Raton, Fla.
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
EVALUATION OF A GAMMA-IRRADIATED, GAMMA GLOBULIN COUPLED, PARTIALLY PURIFIED MATERIAL AS AN IMMUNIZING AGENT FOR EQUINE INFECTIOUS ANEMIA (EIA)
Other Title Info
EVALUATION OF A GAMMA-IRRADIATED, GAMMA GLOBULIN COUPLED, PARTIALLY PURIFIED MATERIAL AS AN IMMUNIZING AGENT FOR EQUINE INFECTIOUS ANEMIA (EIA)