Note
Thesis (M.S.)--Florida Atlantic University, 2004.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2004
Description
A novel, 'near-real' time technique; peptide nucleic acid chemilumiscent in situ hybridization (PNA CISH), was developed and validated for detecting, enumerating and identifying potential indicators (Staphyloccus aureus and Pseudomonas aeruginosa) of beach quality. To understand the dynamics of bacterial indicators a plethora of physical, chemical and biological parameters were tested under field and lab conditions. Escherichia coli tagged with green fluorescence protein (GFP) was used to assess the impact of wave energy and rainfall on seawater counts. PNA CISH and plate counts correlated strongly (r = 0.94 to r = 0.86). Temperature, salinity, nutrient availability and predation significantly influenced the survival of indicators. Rainfall and wave energy affected the re-suspension of bacteria from sand onto overlying water. Overall PNA CISH provides a reliable rapid method for beach monitoring. The implications of beach topography and sampling time on the numbers of Clostridium perfringens and other potential indicators are discussed. Data suggest a revision of policy for tropical sandy beaches.
Language
Type
Form
Extent
151 p.
Subject (Topical)
Identifier
9780496233663
ISBN
9780496233663
Additional Information
Thesis (M.S.)--Florida Atlantic University, 2004.
Date Backup
2004
Date Text
2004
Date Issued (EDTF)
2004
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-18 22:24:14", modified_by="staff:fcllz", modification_date="2011-01-06 13:08:53"
IID
FADT13117
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
Mohammed, Renuka Lisa
Graduate College
Physical Description
151 p.
application/pdf
Title Plain
evaluation of the microbiological quality of sandy beaches: A novel approach
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
2004
monographic
Boca Raton, Fla.
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
evaluation of the microbiological quality of sandy beaches: A novel approach
Other Title Info
An
evaluation of the microbiological quality of sandy beaches: A novel approach