Genetic variation amongst different populations of Typha domingensis and Typha latifolia (cattails) in the Florida Everglades

This thesis is intended to explore the genetic variation between cattail species (Typha spp.), within T. domingensis in different locations, anthropoegenic conditions, and possibly discover a hybrid in the Florida Everglades. Typha domingensis is the dominant cattail species in the Everglades, while Typha latifolia a less common species is also present. Five nuclear and chloroplast protein encoding genes from around 20 samples of cattail plants were collected randomly in the Water Conservation Areas of the Everglades Protection Act, cloned and sequenced. The results of sequencing showed differences between the two species studied, using an insertion within an intron of the Type 2 Metallothionein-like protein gene as a marker to differentiate between the two species. A high degree of nucleotide polymorphisms interspecifically was revealed. Species identification based on morphology is not always reliable that is why our marker must be utilized to confirm the identity of a plant.