Note
by Jiesi Xu.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2010
Description
Microcystin-LR (MCLR) is hepatotoxic to animals and humans with disruption of liver structure causing cytoskeletal damage, necrosis and pooling of blood in the liver, leading to large increase in liver weight. It is also a strong liver tumor promoter and protein phosphatase inhibitor. Microcysin-LR binds protein phosphatases 1 and 2A, and influences regulation of cellular protein phosphorylation. In the present study, a colloidal gold based immunoassay test strip was developed for Microcystin-LR detection. The detection limit was found to be 1 ng/mL. 5 nm colloidal gold test strips exhibits more efficient for detection, compared with 20 nm colloidal gold test strips. The interaction between Microcystin-LR antibody (immunoglobulin G) and colloidal gold nanoparticles was investigated by various analytical methods, including Ultraviolet/Visible (UV/VIS), Fourier Transform Infrared (FTIR) and Fluorescence spectroscopy as well as transmission electron microscopy (TEM).
Language
Type
Form
Extent
ix, 53 p. : ill. (some col.)
Subject (Topical)
Identifier
651971675
OCLC Number
651971675
Additional Information
by Jiesi Xu.
Thesis (M.S.)--Florida Atlantic University, 2010.
Includes bibliography.
Electronic reproduction. Boca Raton, Fla., 2010. Mode of access: World Wide Web.
Date Backup
2010
Date Text
2010
Date Issued (EDTF)
2010
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing7156", creator="creator:SPATEL", creation_date="2010-08-03 11:11:22", modified_by="super:SPATEL", modification_date="2012-04-13 13:37:08"
IID
FADT2683532
Organizations
Attributed name: Charles E. Schmidt College of Science
Attributed name: Department of Chemistry and Biochemistry
Person Preferred Name
Xu, Jiesi.
Graduate College
Physical Description
electronic
ix, 53 p. : ill. (some col.)
Title Plain
Immunoassay test strip for Microcystin-LR detection
Use and Reproduction
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
Boca Raton, Fla.
Florida Atlantic University
2010
Place
Boca Raton, Fla.
Title
Immunoassay test strip for Microcystin-LR detection
Other Title Info
Immunoassay test strip for Microcystin-LR detection