Note
by Amber Butyn.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2009
Description
Talin is a ubiquitous, high-molecular-weight, flexible protein that plays a critical role in focal adhesions by activating, as well as connecting, integrins to the actin cytoskeleton. Talin's inactive auto-inhibitory state is speculated to be one of its modes of regulation inside the cell and is achieved through its head-tail interactions. In order to decipher the stability of this interaction, the head domain (residues 206-405) was cloned into a modified pET28m vector while the tail domains (residues 1654-2344 and 2225-2344) were cloned into the pET32a vector to obtain octa-histidine tagged and un-tagged peptide, respectively. Neither co-expression nor pull-down using the His-tagged head domain was successful in purifying a stable head-tail complex. Our results indicate rather weak interactions between the talin head and rod domains and hence, under our experimental conditions, do not lead to a stable auto-inhibitory complex that can be purified for further studies.
Language
Type
Genre
Extent
vi, 35 leaves : ill. (some col.) ; 29 cm.
Subject (Topical)
Identifier
459787318
OCLC Number
459787318
Rights
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Additional Information
by Amber Butyn.
Thesis (B.A.)--Florida Atlantic University, Honors College, 2009.
Bibliography: leaves 32-35.
Electronic reproduction. Boca Raton, Fla., 2009. Mode of access: World Wide Web.
Date Backup
2009
Date Text
2009
Date Issued (EDTF)
2009
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing3854", creator="creator:SPATEL", creation_date="2009-06-08 13:38:00", modified_by="super:FAUDIG", modification_date="2012-06-05 10:14:01"
IID
FADT209984
Issuance
multipart monograph
Organizations
Attributed name: Harriet L. Wilkes Honors College
Person Preferred Name
Butyn, Amber.
Physical Description
electronic
electronic resource
vi, 35 leaves : ill. (some col.) ; 29 cm.
Title Plain
Investigation of talin head-tail interactions
Use and Reproduction
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Origin Information
2009
multipart monograph
Florida Atlantic University
Physical Location
FBoU FAUER
Title
Investigation of talin head-tail interactions
Other Title Info
Investigation of talin head-tail interactions