The carbon framework of all polyketides is assembled by a polyketide synthase (PKS). Previously, PKS encoding genes were amplified from K. brevis culture and their similarity to a PKS gene from the closely related protist, Cryptosporidium parvum, suggested that these genes originate from the dinoflagellate. However, K. brevis has not been grown axenically. The associated bacteria might be the source of the toxins or the PKS genes. Herein we report the localization of PKS encoding genes by a combination of flow cytometry/PCR and fluorescence in situ hybridization (FISH).
This is the author’s version of a work accepted for publication byElsevier. Changes resulting from the publishing process, including peer review, editing,corrections, structural formatting and other quality control mechanisms, may not be reflectedin this document. Changes may have been made to this work since it was submitted forpublication. The definitive version has been published athttp://www.elsevier.com/locate/phytochem and may be cited as Snyder, Richard V., MariaA. Guerrero, Christopher D. Sinigalliano, Jamie Winshell, Roberto Perez, Jose V. Lopez,Kathleen S. Rein (2005) Localization of polyketide synthase encoding genes to the toxicdinoflagellate Karenia brevis, Phytochemistry 66: 1767-1780 doi:10.1016/j.phytochem.2005.06.010
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