Note
A modification of the Bligh and Dyer lipid extraction method (Canadian Journal of Biochemistry and Physiology, 37, 911–917, 1959) is described. The technique utilizes commercially available Teflon membrane microfiltration units that permit filtration and solvent separation to occur simultaneously. Thus, rapid and efficient microextraction of total lipid (20–200 μg) can be accomplished from tissue homogenates at reduced solvent volumes. The primary advantages of this modification include increased accuracy for small samples, increased productivity and reduced exposure to solvent fumes.