Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2017
EDTF Date Created
2017
Description
DNA is damaged by many environmental factors like ultraviolet light from the
sun and even by by-products of normal metabolism in our bodies, for example reactive
oxygen species. A mouse model was used to test the effects DNA damage has on aging
and age-related diseases. Damaged DNA is an early sign of muscle aging. The ERCC1-
XPF complex is important because it repairs DNA. Using the Cre-Lox system, we
knocked-out the Ercc1 gene in differentiated myocytes or muscle fibers. We measured
body weight, fat content, muscle strength, and the ability of muscle to break down
glucose, and found no comparable differences between the mutant and wild type mice.
The histology also showed no significant difference between the two. These knockout
mice only lived to be four to six months old, as oppose to the wild-type mice, which can
live up to 35 months. Surprisingly, they died prematurely of heart disease. This
demonstrates that cardiac muscle is more sensitive to DNA damage than skeletal muscle.
sun and even by by-products of normal metabolism in our bodies, for example reactive
oxygen species. A mouse model was used to test the effects DNA damage has on aging
and age-related diseases. Damaged DNA is an early sign of muscle aging. The ERCC1-
XPF complex is important because it repairs DNA. Using the Cre-Lox system, we
knocked-out the Ercc1 gene in differentiated myocytes or muscle fibers. We measured
body weight, fat content, muscle strength, and the ability of muscle to break down
glucose, and found no comparable differences between the mutant and wild type mice.
The histology also showed no significant difference between the two. These knockout
mice only lived to be four to six months old, as oppose to the wild-type mice, which can
live up to 35 months. Surprisingly, they died prematurely of heart disease. This
demonstrates that cardiac muscle is more sensitive to DNA damage than skeletal muscle.
Language
Type
Genre
Form
Extent
23 p.
Identifier
FA00012622
Additional Information
Includes bibliography.
Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2017.
FAU Honors Theses Digital Collection
Date Backup
2017
Date Created Backup
2017
Date Text
2017
Date Created (EDTF)
2017
Date Issued (EDTF)
2017
Extension
FAU
IID
FA00012622
Organizations
Attributed name: Florida Atlantic University
Attributed name: Harriet L. Wilkes Honors College
Person Preferred Name
Hennessy-Wack, Danielle
author
Physical Description
application/pdf
23 p.
Title Plain
PHENOTYPIC CHARACTERIZATION OF MICE LACKING DNA REPAIR IN THE MUSCLE
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Origin Information
2017
2017
Florida Atlantic University
Jupiter, Florida
Physical Location
Florida Atlantic University Libraries
Place
Jupiter, Florida
Sub Location
Digital Library
Title
PHENOTYPIC CHARACTERIZATION OF MICE LACKING DNA REPAIR IN THE MUSCLE
Other Title Info
PHENOTYPIC CHARACTERIZATION OF MICE LACKING DNA REPAIR IN THE MUSCLE