Kelly-Borges et al. (1991) and Kelly-Borges & Pomponi (1994) utilised partial 18S rRNA
gene sequences to resolve relationships within hadromerid and lithistid sponges (Porifera:
Demospongiae). While their results clarified several specific systematic problems, their
conclusions were hampered by low levels of sequence variation. This study sought primarily
to evaluate the resolution potential between regions of the 18S rRNA gene used in previous
studies on sponges, and 28S rRNA genes used in more recent work. Six lithistid sponge taxa
were chosen to represent a gradient of taxonomic relationships, ranging through genus,
family, order and class. Approximately I ,300bp of the 18S rRNA gene and a 700bp region at
the 5' end of the 28S rRNA gene were compared with the data ofKelly-Borges & Pomponi
(1994). We found that the 700bp region of the 28S rRNA gene presented the greatest
potential for resolution of this group of Porifera at the genus and family level, and that the
resultant molecular phylogeny was congruent with morphological hypotheses for the group.
Member of
Contributors
Publisher
Queensland Museum
Date Issued
1999
Note
Language
Type
Genre
Form
Extent
10 p.
Subject (Topical)
Identifier
FA00007274
Additional Information
Kelly-Borges et al. (1991) and Kelly-Borges & Pomponi (1994) utilised partial 18S rRNA
gene sequences to resolve relationships within hadromerid and lithistid sponges (Porifera:
Demospongiae). While their results clarified several specific systematic problems, their
conclusions were hampered by low levels of sequence variation. This study sought primarily
to evaluate the resolution potential between regions of the 18S rRNA gene used in previous
studies on sponges, and 28S rRNA genes used in more recent work. Six lithistid sponge taxa
were chosen to represent a gradient of taxonomic relationships, ranging through genus,
family, order and class. Approximately I ,300bp of the 18S rRNA gene and a 700bp region at
the 5' end of the 28S rRNA gene were compared with the data ofKelly-Borges & Pomponi
(1994). We found that the 700bp region of the 28S rRNA gene presented the greatest
potential for resolution of this group of Porifera at the genus and family level, and that the
resultant molecular phylogeny was congruent with morphological hypotheses for the group.
gene sequences to resolve relationships within hadromerid and lithistid sponges (Porifera:
Demospongiae). While their results clarified several specific systematic problems, their
conclusions were hampered by low levels of sequence variation. This study sought primarily
to evaluate the resolution potential between regions of the 18S rRNA gene used in previous
studies on sponges, and 28S rRNA genes used in more recent work. Six lithistid sponge taxa
were chosen to represent a gradient of taxonomic relationships, ranging through genus,
family, order and class. Approximately I ,300bp of the 18S rRNA gene and a 700bp region at
the 5' end of the 28S rRNA gene were compared with the data ofKelly-Borges & Pomponi
(1994). We found that the 700bp region of the 28S rRNA gene presented the greatest
potential for resolution of this group of Porifera at the genus and family level, and that the
resultant molecular phylogeny was congruent with morphological hypotheses for the group.
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution 1292
This manuscript is an author version with the final publication
available and may be cited as: McInerney, J. O., Adams, C. L., & Kelly, M. (1999). Phylogenetic reso
lution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida. Memoirs of the
Queensland Museum 44, 343-351.
available and may be cited as: McInerney, J. O., Adams, C. L., & Kelly, M. (1999). Phylogenetic reso
lution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida. Memoirs of the
Queensland Museum 44, 343-351.
Date Backup
1999
Date Text
1999
Date Issued (EDTF)
1999
Extension
FAU
IID
FA00007274
Organizations
Attributed name: Kelly-Borges, M.
Attributed name: Harbor Branch Oceanographic Institute
Person Preferred Name
McInerney, James O.
Physical Description
10 p.
Title Plain
Phylogenetic resolution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida.
Origin Information
1999
Queensland Museum
Brisbane
Place
Brisbane
Title
Phylogenetic resolution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida.
Other Title Info
Phylogenetic resolution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida.