Probiotics in aquaculture: An analysis of the bacteria associated with the gut of healthy shrimps, Penaeus spp

A recent surge in the consumption of shrimps has compelled the aquaculture industry to expand greatly. Bacteriosis poses a major challenge to shrimp cultivation. Probiotic control of disease in larvicultures has been on the rise for the past decade; however, there has been little research to provide required frameworks for this promising technology. In this study, the normal gut flora of the commercially important shrimp, Penaeus vannamei, and its relative, P. duorarum was extensively analyzed to provide a basis for selection of putative probiotic strains. Heterotorphic members of the gut and ambient water were isolated and characterized by culture techniques, microscopy, Biolog biochemical fingerprinting, whole cell crude protein fingerprinting, and 16S rDNA sequence analysis. Non-pathogenic Vibrio harveyi-like strains were prevalent in the gut of both penaied shrimps, regardless of age and source. Community level physiological profiling by principal component analysis showed the gut community was fairly stable throughout shrimp life stages.