Note
Thesis (M.S.)--Florida Atlantic University, 1972.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
1972
Description
Cellulolytic enzymes were induced in the marine fungal isolates,
Culcitalna achraspora, Halosphaeria mediosetigera, and Zalerion
xylestrix by native cotton and related substrates. Marine
environmental conditions were maintained in an artificial
seawater medium. Induced c1 and ex enzymes and cellobiase were
determined colorimetrically. Induction also occurred on
cellobiose, but not on glucose and lactose. Permenter studies
with glucose and with toilet tissue carbon sources gave close
correlations between mycelial dry weight, enzyme component
activity, and cell nitrogen. In cotton substrate media,
Tween 80 increased C1 activity by 148%, and Cx activity by
160%, on the average. Millipore ultrafiltration was the best
technique for concentrating cellulase for Sephadex G-75 column
chromatography, while conventional techniques were unsuccessful.
Fractionation diagrams for all cultures were similar with
2 distinct peaks. The low molecular weight fraction contained
mainly Cx enzyme activity, while the high molecular weight
fraction contained C1 and Cx enzymes and cellobiase. These
fungi possess a cellulase similar, but not identical, to that
found in terrestrial species.
Culcitalna achraspora, Halosphaeria mediosetigera, and Zalerion
xylestrix by native cotton and related substrates. Marine
environmental conditions were maintained in an artificial
seawater medium. Induced c1 and ex enzymes and cellobiase were
determined colorimetrically. Induction also occurred on
cellobiose, but not on glucose and lactose. Permenter studies
with glucose and with toilet tissue carbon sources gave close
correlations between mycelial dry weight, enzyme component
activity, and cell nitrogen. In cotton substrate media,
Tween 80 increased C1 activity by 148%, and Cx activity by
160%, on the average. Millipore ultrafiltration was the best
technique for concentrating cellulase for Sephadex G-75 column
chromatography, while conventional techniques were unsuccessful.
Fractionation diagrams for all cultures were similar with
2 distinct peaks. The low molecular weight fraction contained
mainly Cx enzyme activity, while the high molecular weight
fraction contained C1 and Cx enzymes and cellobiase. These
fungi possess a cellulase similar, but not identical, to that
found in terrestrial species.
Language
Type
Form
Extent
73 p.
Subject (Topical)
Identifier
13537
Additional Information
Thesis (M.S.)--Florida Atlantic University, 1972.
Date Backup
1972
Date Text
1972
Date Issued (EDTF)
1972
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-19 01:14:12", modified_by="staff:fcllz", modification_date="2011-01-06 13:08:57"
IID
FADT13537
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
WHITE, JAMES L., JR.
Graduate College
Physical Description
73 p.
application/pdf
Title Plain
PRODUCTION, INDUCTION, AND RESOLUTION OF THE CELLULOLYTIC ENZYME COMPLEX IN SELECTED FILAMENTOUS MARINE FUNGI
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
1972
monographic
Boca Raton, Fla.
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
PRODUCTION, INDUCTION, AND RESOLUTION OF THE CELLULOLYTIC ENZYME COMPLEX IN SELECTED FILAMENTOUS MARINE FUNGI
Other Title Info
PRODUCTION, INDUCTION, AND RESOLUTION OF THE CELLULOLYTIC ENZYME COMPLEX IN SELECTED FILAMENTOUS MARINE FUNGI