A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells

Member of
HBOI Faculty Contributions
Contributors
Longley, Ross E.
Harmody, Dedra K.
Publisher
Nature Publishing Group
Date Issued
1991
Note

A rapid, colorimetric, microassay for detection of agents which are known agonists/antagonists of protein kinase C (PKC) was developed, utilizing their effects on adherence of EL-4.IL-2 cells. Cells that were incubated with agents which are known inducers of PKC activation, phorbol 12-myristate 13-acetate (PMA), phorbol 12, 13-dibutyrate (PDBu), mezerein and indolactam V, readily adhered to wells of 96 well microtiter plates within 1 - 2 hours, whereas cells incubated with the negative PKC activator, 4α-phorbol 12-myristate 13-acetate (4α-PMA), which is structurally related to PMA (4β-PMA), did not adhere.

Language
English
Type
Text
Genre
article
Extent
11 p.
Subject (Topical)
Protein kinase C
Colorimetric analysis
Diglycerides
Identifier
3351944
Additional Information
A rapid, colorimetric, microassay for detection of agents which are known agonists/antagonists of protein kinase C (PKC) was developed, utilizing their effects on adherence of EL-4.IL-2 cells. Cells that were incubated with agents which are known inducers of PKC activation, phorbol 12-myristate 13-acetate (PMA), phorbol 12, 13-dibutyrate (PDBu), mezerein and indolactam V, readily adhered to wells of 96 well microtiter plates within 1 - 2 hours, whereas cells incubated with the negative PKC activator, 4α-phorbol 12-myristate 13-acetate (4α-PMA), which is structurally related to PMA (4β-PMA), did not adhere.
This manuscript is an author version with the final publication available at http://www.nature.com/ja/index.html and may be cited as: Longley R. E., & Harmody, D. (1991). A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells. The Journal of Antibiotics, 44(1), 93-102.
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #818.
Date Backup
1991
Date Text
1991
Date Issued (EDTF)
1991
Extension


FAU
FAU
admin_unit="FAU01", ingest_id="ing13500", creator="creator:BCHANG", creation_date="2012-08-10 17:15:40", modified_by="super:FAUDIG", modification_date="2014-02-14 13:01:54"

IID
FADT3351944
Issuance
single unit
Organizations
Attributed name: Harbor Branch Oceanographic Institute
Person Preferred Name

Longley, Ross E.

creator

Physical Description

pdf
11 p.
PURL
http://purl.flvc.org/FCLA/DT/3351944
Title Plain
A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells
URL
http://purl.flvc.org/FCLA/DT/3351944
Origin Information

Nature Publishing Group
1991
single unit
Title
A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells
Other Title Info

A rapid colorimetric microassay to detect agonists/antagonists of protein kinase C based on adherence of EL-4.IL-2 cells