A simple colorimetric screening procedure using the tetrazolium salt, MTT, has been adapted for use in determining the cytotoxic effects of agents to nonproliferating cells from murine-derived, primary lymphocyte cultures. Moreover, cytotoxicity from short-term exposure to test compounds is assessed rapidly by this technique. The formazan crystals produced from each culture, a byproduct of the enzymatic conversation of MTT by the mitochondria of living cells, are solubilized and read on a spectrophotometer. This assay is used to differentiate the possible immediate cytotoxic effect of a test compound from a noncytotoxic, immunosuppressive effect as determined by other means. The general application for this procedure is for a rapid assessment of the viability of resting cell cultures by nonvisual, nonradioisotopic means.
Member of
Contributors
Publisher
Springer
Date Issued
1988
Note
Language
Type
Genre
Extent
6 p.
Subject (Topical)
Identifier
3352201
Additional Information
A simple colorimetric screening procedure using the tetrazolium salt, MTT, has been adapted for use in determining the cytotoxic effects of agents to nonproliferating cells from murine-derived, primary lymphocyte cultures. Moreover, cytotoxicity from short-term exposure to test compounds is assessed rapidly by this technique. The formazan crystals produced from each culture, a byproduct of the enzymatic conversation of MTT by the mitochondria of living cells, are solubilized and read on a spectrophotometer. This assay is used to differentiate the possible immediate cytotoxic effect of a test compound from a noncytotoxic, immunosuppressive effect as determined by other means. The general application for this procedure is for a rapid assessment of the viability of resting cell cultures by nonvisual, nonradioisotopic means.
This manuscript is an author version with the final publication available at http://www.springerlink.com/ and may be cited as: Faircloth, G. T., Stewart, D., & Clement, J. J. (1988). A simple screening procedure for the quantitative measurement of cytotoxicity to resting primary lymphocyte cultures. Methods in Cell Science [formerly Journal of Tissue Culture Methods], 11(4), 201-205. doi:10.1007/BF01407314
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #697.
Date Backup
1988
Date Text
1988
DOI
10.1007/BF01407314
Date Issued (EDTF)
1988
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing13603", creator="creator:BCHANG", creation_date="2012-08-30 16:56:28", modified_by="super:FAUDIG", modification_date="2014-02-14 15:52:54"
IID
FADT3352201
Issuance
single unit
Organizations
Attributed name: Harbor Branch Oceanographic Institute
Person Preferred Name
Faircloth, G. T.
creator
Physical Description
6 p.
Title Plain
A simple screening procedure for the quantitative measurement of cytotoxicity to resting primary lymphocyte cultures
Origin Information
Springer
1988
single unit
Title
A simple screening procedure for the quantitative measurement of cytotoxicity to resting primary lymphocyte cultures
Other Title Info
A simple screening procedure for the quantitative measurement of cytotoxicity to resting primary lymphocyte cultures