Primary cell cultures of several species of shallow water marine demosponges have been initiatedto determine the feasibility of cell culture for bulk production of bioactive secondary metabolites. Selective cellenrichment and determination of cell-type specific localization of bioactive metabolites are accomplished bydensity gradient separation and chromatographic analyses of cell fractions. Microbial contamination has beencontrolled through the useofantibiotics . Analysis of dose-responsecurves for inhibition of microbial contaminationand effect on sponge cell viability has resulted in establishment of optimal antibiotic concentrations for cultureinitiation and maintenance. Physiological responses of cells are monitored by direct microscopic analysis, chromatographictechniques, colorimetric bioassays, and flow cytometric analyses of cell constituents and functions .Results of culture optimization studies to enhance biomass andlormetabolite production in Hymeniacidon heliophilaindicate that doubling rates can be significantly increased in response to mitogens, and cells will continue toproduce secondary metabolites in culture. These results suggest that sponge cell culture may be feasible for bulkproduction of bioactive secondary metabolites.
Member of
Contributors
Publisher
A. A. Balkema.
Date Issued
1994
Note
Language
Type
Genre
Form
Extent
8 p.
Subject (Topical)
Identifier
FA00007381
Additional Information
Primary cell cultures of several species of shallow water marine demosponges have been initiatedto determine the feasibility of cell culture for bulk production of bioactive secondary metabolites. Selective cellenrichment and determination of cell-type specific localization of bioactive metabolites are accomplished bydensity gradient separation and chromatographic analyses of cell fractions. Microbial contamination has beencontrolled through the useofantibiotics . Analysis of dose-responsecurves for inhibition of microbial contaminationand effect on sponge cell viability has resulted in establishment of optimal antibiotic concentrations for cultureinitiation and maintenance. Physiological responses of cells are monitored by direct microscopic analysis, chromatographictechniques, colorimetric bioassays, and flow cytometric analyses of cell constituents and functions .Results of culture optimization studies to enhance biomass andlormetabolite production in Hymeniacidon heliophilaindicate that doubling rates can be significantly increased in response to mitogens, and cells will continue toproduce secondary metabolites in culture. These results suggest that sponge cell culture may be feasible for bulkproduction of bioactive secondary metabolites.
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution 985
This manuscript is an author version with the final publication available
and may be cited as: Pomponi, S. A., & Willoughby, R. (1994). Sponge cell culture for production of
bioactive metabolites. In R. W. M. van Soest, T. M. G. van Kempen, & J. C. Braekman (Eds.), Sponges
in Time and Space: Biology, Chemistry, Paleontology: proceedings of the 4th International Porifera
Congress, Amsterdam, Netherlands, 19-23 April 1993 (pp. 395-400). Rotterdam; Brookfield, VT: A. A.
Balkema.
and may be cited as: Pomponi, S. A., & Willoughby, R. (1994). Sponge cell culture for production of
bioactive metabolites. In R. W. M. van Soest, T. M. G. van Kempen, & J. C. Braekman (Eds.), Sponges
in Time and Space: Biology, Chemistry, Paleontology: proceedings of the 4th International Porifera
Congress, Amsterdam, Netherlands, 19-23 April 1993 (pp. 395-400). Rotterdam; Brookfield, VT: A. A.
Balkema.
Date Backup
1994
Date Text
1994
Date Issued (EDTF)
1994
Extension
FAU
IID
FA00007381
Person Preferred Name
Pomponi, Shirley A.
spomponi@fau.edu
Physical Description
8 p.
Title Plain
Sponge cell culture for production of bioactive metabolites.
Origin Information
1994
A. A. Balkema.
Brookfield, VT
Place
Brookfield, VT
Title
Sponge cell culture for production of bioactive metabolites.
Other Title Info
Sponge cell culture for production of bioactive metabolites.