Strategies for flow cytometric analysis of marine microalgae and sponge cells

Member of
HBOI Faculty Contributions
Contributors
Yentsch, C. M.
Pomponi, Shirley A.
Publisher
Academic Press, Inc.
Date Issued
1994
Note

The objective of this chapter is to detail two strategies important in order to overcome constraints inherent in flow cytometric analysis of marine microalgae and sponge cells. The two strategies are: (I) selective cell enrichment, employing physical separation techniques, and (2) selective signal enhancement, optimizing the optics. Specifically, we detail cell dissociation and density gradient separation of sponge cells, concentration of microalgae from seawater samples, determination of absolute (vs relative) abundance of microalgal subpopulations, and a metabolic index protocol that can be used for both microalgae and sponge cells.

Language
English
Type
Text
Genre
article
Extent
17 p.
Subject (Topical)
Microalgae
Sponges
Cytometric cellular analysis
Identifier
3353886
Additional Information
The objective of this chapter is to detail two strategies important in order to overcome constraints inherent in flow cytometric analysis of marine microalgae and sponge cells. The two strategies are: (I) selective cell enrichment, employing physical separation techniques, and (2) selective signal enhancement, optimizing the optics. Specifically, we detail cell dissociation and density gradient separation of sponge cells, concentration of microalgae from seawater samples, determination of absolute (vs relative) abundance of microalgal subpopulations, and a metabolic index protocol that can be used for both microalgae and sponge cells.
The final published version of this manuscript is available at http://www.sciencedirect.com/science/bookseries/0091679X and may be cited as: Yentsch, C. M., & Pomponi, S. A. (1994). Strategies for flow cytometric analysis of marine microalgae and sponge cells. In Z. Darzynkiewicz, J. P. Robinson & H. A. Crissman (eds.). Methods in Cell Biology, Part B, 42, 523-538. doi:10.1016/S0091-679X(08)61093-6
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1039.
Date Backup
1994
Date Text
1994
DOI
10.1016/S0091-679X(08)61093-6
Date Issued (EDTF)
1994
Extension


FAU
FAU
admin_unit="FAU01", ingest_id="ing13973", creator="creator:BCHANG", creation_date="2012-10-29 12:44:29", modified_by="super:FAUDIG", modification_date="2014-02-07 16:14:37"

IID
FADT3353886
Issuance
single unit
Person Preferred Name

Yentsch, C. M.

creator

Physical Description

pdf
17 p.
PURL
http://purl.flvc.org/FCLA/DT/3353886
Title Plain
Strategies for flow cytometric analysis of marine microalgae and sponge cells
URL
http://purl.flvc.org/FCLA/DT/3353886
Origin Information

Academic Press, Inc.
1994
single unit
Title
Strategies for flow cytometric analysis of marine microalgae and sponge cells
Other Title Info

Strategies for flow cytometric analysis of marine microalgae and sponge cells