Note
Thesis (M.S.)--Florida Atlantic University, 1977.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
1977
Description
Polyethylene glycol (PEG)-induced aggregation, hemolysis and
fusion of chicken red blood ceils (CRBC's) was studied in relation to
the following parameters: osmotic pressure, polymer concentration,
ionic strength, electrolyte species, surface charge removal and
glutaraldehyde fixation. Indirect evidence indicated that osmotic
pressure does not play a primary role in aggregation and fusion of red
blood cells. Maximum aggregation and fusion was obtained in solutions
of 20 and 40% PEG, respectively. In contrast, hemolysis increased
almost linearily with PEG concentration. The negative electrostatic
charge due to the presence of sialic acid on the membrane surface was
found to be the primary factor affecting aggregation, hemolysis and
fusion. Removal of the charge by neuraminidase or screening with cations
enhanced aggregation and fusion while inhibiting hemolysis. The
inhibition of aggregation by glutaraldehyde fixation, and hemolysis
and fusion results not attributed to surface charges, are discussed
in terms of intramembrane interactions with PEG.
fusion of chicken red blood ceils (CRBC's) was studied in relation to
the following parameters: osmotic pressure, polymer concentration,
ionic strength, electrolyte species, surface charge removal and
glutaraldehyde fixation. Indirect evidence indicated that osmotic
pressure does not play a primary role in aggregation and fusion of red
blood cells. Maximum aggregation and fusion was obtained in solutions
of 20 and 40% PEG, respectively. In contrast, hemolysis increased
almost linearily with PEG concentration. The negative electrostatic
charge due to the presence of sialic acid on the membrane surface was
found to be the primary factor affecting aggregation, hemolysis and
fusion. Removal of the charge by neuraminidase or screening with cations
enhanced aggregation and fusion while inhibiting hemolysis. The
inhibition of aggregation by glutaraldehyde fixation, and hemolysis
and fusion results not attributed to surface charges, are discussed
in terms of intramembrane interactions with PEG.
Language
Type
Form
Extent
73 p.
Subject (Topical)
Identifier
13893
Additional Information
Thesis (M.S.)--Florida Atlantic University, 1977.
Charles E. Schmidt College of Science
FAU Electronic Theses and Dissertations Collection
Date Backup
1977
Date Text
1977
Date Issued (EDTF)
1977
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-19 01:47:15", modified_by="staff:fcllz", modification_date="2011-01-06 13:09:03"
IID
FADT13893
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
BATCHELOR, ROGER ALLEN.
Graduate College
Physical Description
73 p.
application/pdf
Title Plain
STUDIES ON POLYETHYLENE GLYCOL-MEDIATED AGGREGATION AND FUSION OF AVIAN ERYTHROCYTES
Use and Reproduction
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Origin Information
1977
monographic
Boca Raton, Fla.
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
STUDIES ON POLYETHYLENE GLYCOL-MEDIATED AGGREGATION AND FUSION OF AVIAN ERYTHROCYTES
Other Title Info
STUDIES ON POLYETHYLENE GLYCOL-MEDIATED AGGREGATION AND FUSION OF AVIAN ERYTHROCYTES