Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2016
EDTF Date Created
2016
Description
Oxidative stress (OS) is strongly implicated in age-related neurodegeneration and
other diseases. Under OS, the production of excessive oxidants leads to increased
damages to cellular components. Recently, RNA has been discovered as a major target of
oxidative damage, including the creation of abasic sites. In this work, we developed a
method for quantifying abasic RNA in cell. Using this method, we have examined the
potential role of the RNA-processing cellular foci, stress granule (SG) and processing
bodies (PB) in eliminating abasic RNA in situ. We demonstrated that RNA is a major
target of oxidative damage, constituting the majority of OS-induced abasic nucleic acids
in HeLa cell. Importantly, the level of abasic RNA is strongly correlated with SG
abundance. Furthermore, inhibition of SG/PB formation causes accumulation of abasic
RNA, suggesting that SG/PB participates in removing oxidized RNA and protects cells
under OS, which offers novel targets for therapeutic intervention in age-related diseases.
other diseases. Under OS, the production of excessive oxidants leads to increased
damages to cellular components. Recently, RNA has been discovered as a major target of
oxidative damage, including the creation of abasic sites. In this work, we developed a
method for quantifying abasic RNA in cell. Using this method, we have examined the
potential role of the RNA-processing cellular foci, stress granule (SG) and processing
bodies (PB) in eliminating abasic RNA in situ. We demonstrated that RNA is a major
target of oxidative damage, constituting the majority of OS-induced abasic nucleic acids
in HeLa cell. Importantly, the level of abasic RNA is strongly correlated with SG
abundance. Furthermore, inhibition of SG/PB formation causes accumulation of abasic
RNA, suggesting that SG/PB participates in removing oxidized RNA and protects cells
under OS, which offers novel targets for therapeutic intervention in age-related diseases.
Language
Type
Form
Extent
94 p.
Subject (Topical)
Identifier
FA00004702
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2016.
FAU Electronic Theses and Dissertations Collection
Date Backup
2016
Date Created Backup
2016
Date Text
2016
Date Created (EDTF)
2016
Date Issued (EDTF)
2016
Extension
FAU
IID
FA00004702
Organizations
Attributed name: Florida Atlantic University
Attributed name: Charles E. Schmidt College of Medicine
Attributed name: Department of Biomedical Science
Person Preferred Name
Pourkalbassi, Delaram
author
Graduate College
Physical Description
application/pdf
94 p.
Title Plain
A Study on the Potential Role of Stress Granules and Processing Bodies in Eliminating Oxidatively Damaged RNA
Use and Reproduction
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Origin Information
2016
2016
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
A Study on the Potential Role of Stress Granules and Processing Bodies in Eliminating Oxidatively Damaged RNA
Other Title Info
A Study on the Potential Role of Stress Granules and Processing Bodies in Eliminating Oxidatively Damaged RNA