Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2019
EDTF Date Created
2019
Description
The Supported red blood cell membrane (SRBCm) was developed on a piezoelectric sensor to study the attachment of nanoparticles to erythrocyte surfaces. A well-dispersed colloidal suspension of fragments of RBCm was prepared from whole blood, and characterized thoroughly using cryogenic transmission electron microscopy, dynamic light scattering, and zeta potential analysis. To develop SRBCm, RBCm fragments were immobilized onthe sensor in a quartz crystal microbalance with dissipation monitoring system. A complete monolayer of flattened fragments of RBCm was formed on the positively charged surface of the piezoelectric sensor in 1 mM NaCl and 0.2 mM NaHCO3 at pH 7.1. The surface morphology of SRBCm was characterized via atomic force microscopy. The even distribution of surface proteins expressed on erythrocytes was found on SRBCm through indirect immunofluorescence microscopy. The attachment efficiencies of model nanoparticles, e.g. hematite nanoparticles and carboxylated polystyrene nanoparticles, on the SRBCm were quantified using a classic methodology.
KEYWORDS: Supported erythrocyte membrane, piezoelectric sensor, phospholipid bilayers, nanoparticles
KEYWORDS: Supported erythrocyte membrane, piezoelectric sensor, phospholipid bilayers, nanoparticles
Language
Type
Form
Extent
86 p.
Subject (Topical)
Identifier
FA00013383
Rights
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2019.
FAU Electronic Theses and Dissertations Collection
Date Backup
2019
Date Created Backup
2019
Date Text
2019
Date Created (EDTF)
2019
Date Issued (EDTF)
2019
Extension
FAU
IID
FA00013383
Organizations
Attributed name: Florida Atlantic University
Attributed name: Department of Civil, Environmental and Geomatics Engineering
Attributed name: College of Engineering and Computer Science
Person Preferred Name
Islam, Tanaz
author
Graduate College
Physical Description
application/pdf
86 p.
Title Plain
SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
2019
2019
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS
Other Title Info
SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS