Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2008
EDTF Date Created
2008
Description
In spite the heavy investments in therapeutic research breast cancer still impacts the
lives of women globally. The projected incidence of new cases in USA for 2008 is 67,770,
with estimated 40,480 deaths. In this study, we investigated the therapeutic efficacy of
Cytoreg®-genistein combination treatment on MCF-7 human breast cancer cells. MCF-7
cells were treated with genistein and Cytoreg® single and combination treatments for 24-
48hr; and the chemosensitivity assessed using bioassays: Trypan Blue and MTT for cell
viability; Ethidium bromide/Rhodamine 123 to assess apoptosis induction; F AM PolyCaspase
binding assay for mechanism of action. The overall data indicated dose- and timedependent
cell death in the MCF-cells and that apoptosis was the major means of treatmentinduced
growth inhibition. There was evidence of Cytoreg®-induced autophagy in the cells.
The overall findings indicated that genistein-Cytoreg® combination was more efficacious
than either genistein or Cytoreg® alone. Cytoreg® enhanced the phytosensitivity of MCF-7
cells to genistein isoflavone.
lives of women globally. The projected incidence of new cases in USA for 2008 is 67,770,
with estimated 40,480 deaths. In this study, we investigated the therapeutic efficacy of
Cytoreg®-genistein combination treatment on MCF-7 human breast cancer cells. MCF-7
cells were treated with genistein and Cytoreg® single and combination treatments for 24-
48hr; and the chemosensitivity assessed using bioassays: Trypan Blue and MTT for cell
viability; Ethidium bromide/Rhodamine 123 to assess apoptosis induction; F AM PolyCaspase
binding assay for mechanism of action. The overall data indicated dose- and timedependent
cell death in the MCF-cells and that apoptosis was the major means of treatmentinduced
growth inhibition. There was evidence of Cytoreg®-induced autophagy in the cells.
The overall findings indicated that genistein-Cytoreg® combination was more efficacious
than either genistein or Cytoreg® alone. Cytoreg® enhanced the phytosensitivity of MCF-7
cells to genistein isoflavone.
Language
Type
Form
Extent
67 p.
Subject (Topical)
Identifier
FA00000777
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2008.
Charles E. Schmidt College of Science
FAU Electronic Theses and Dissertations Collection
Date Backup
2008
Date Created Backup
2008
Date Text
2008
Date Created (EDTF)
2008
Date Issued (EDTF)
2008
Extension
FAU
FAU
IID
FA00000777
Organizations
Attributed name: Florida Atlantic University
Attributed name: Charles E. Schmidt College of Science
Attributed name: Department of Biological Sciences
Person Preferred Name
Johnson, Michelle M.
Graduate College
Physical Description
application/pdf
67 p.
Title Plain
Therapeutic Options for the Treatment of Breast Cancer: Using Cytoreg and Genistein Isoflavone
Use and Reproduction
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Origin Information
2008
2008
Florida Atlantic University
Boca Raton, FL
Physical Location
Florida Atlantic University Digital Library
Place
Boca Raton, FL
Sub Location
Boca Raton, Fla.
Digital Library
Title
Therapeutic Options for the Treatment of Breast Cancer: Using Cytoreg and Genistein Isoflavone
Other Title Info
Therapeutic Options for the Treatment of Breast Cancer: Using Cytoreg and Genistein Isoflavone