Note
Thesis (M.S.)--Florida Atlantic University, 2004.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2004
Description
The therapeutic potential of pomegranate and genistein on growth inhibition of human breast cancer cells was investigated. Methods. Cells (MCF-7) were initially cultured for 48 hr to achieve 80% confluence; and then exposed to the agents in single and combination treatments. Post-treatment analysis was done by using a series of bioassays, including LDH, MTS, AcrO-EthBr, Annexin-FITC and TUNEL assays for growth inhibition and apoptosis detection; and Caspase-3 and NQO1 for molecular pathways of apoptosis. Results. Pomegranate and genistein showed significant dose- and time-dependent cytotoxic and growth inhibition effects as well as apoptosis induction in MCF-7 cancer cells, with significantly higher ( P < 0.01) effects in the combination treatments than in the single treatments. Both drugs induced apoptosis through a caspase-mediated mechanism and independent of NQO1. Discussion and conclusions. Pomegranate and genistein inhibit the growth of MCF-7 breast cancer cells through induction of apoptosis with combination treatment being more efficacious than single treatments.
Language
Type
Form
Extent
85 p.
Subject (Topical)
Identifier
9780496239382
ISBN
9780496239382
Additional Information
Thesis (M.S.)--Florida Atlantic University, 2004.
Date Backup
2004
Date Text
2004
Date Issued (EDTF)
2004
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-18 22:25:28", modified_by="staff:fcllz", modification_date="2011-01-06 13:08:54"
IID
FADT13130
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
Louis Jeune, Marie Adeline
Graduate College
Physical Description
85 p.
application/pdf
Title Plain
Therapeutic potential of pomegranate and genistein for human breast cancer
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
2004
monographic
Boca Raton, Fla.
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Therapeutic potential of pomegranate and genistein for human breast cancer
Other Title Info
Therapeutic potential of pomegranate and genistein for human breast cancer