FISH analysis showed a prominent similarity of microbiota in sediments and Geodia spp. samples. Furthermore, the presence of sulfate-reducing and annamox bacteria as well as other obligate anaerobic microorganisms in both Geodia spp. and sediment samples were also confirmed. Anaerobic cultures obtained from the homogenates allowed the isolation of a variety of facultative anaerobes, primarily Bacillus spp. and Vibrio spp. Obligate anaerobes such as Desulfovibrio spp. and Clostridium spp. were also found. We also provide the first evidence for a culturable marine member of the Chloroflexi, which may enter into symbiotic relationships with deep-water sponges such as Geodia spp. Resuspended sediment particles, may provide a source of microorganisms able to associate or form a symbiotic relationship with sponges.
Member of
Publisher
Nature Publishing Group
Date Issued
2010
Note
Language
Type
Genre
Extent
15 p.
Subject (Topical)
Identifier
3351982
Additional Information
FISH analysis showed a prominent similarity of microbiota in sediments and Geodia spp. samples. Furthermore, the presence of sulfate-reducing and annamox bacteria as well as other obligate anaerobic microorganisms in both Geodia spp. and sediment samples were also confirmed. Anaerobic cultures obtained from the homogenates allowed the isolation of a variety of facultative anaerobes, primarily Bacillus spp. and Vibrio spp. Obligate anaerobes such as Desulfovibrio spp. and Clostridium spp. were also found. We also provide the first evidence for a culturable marine member of the Chloroflexi, which may enter into symbiotic relationships with deep-water sponges such as Geodia spp. Resuspended sediment particles, may provide a source of microorganisms able to associate or form a symbiotic relationship with sponges.
This manuscript is an author version with the final publication available at http://www.nature.com/ismej/journal/v4/n5/full/ismej2009149a.html and may be cited as: Bruck, W. M., Bruck, T. B., Self, W. T., Reed, J. K., Nitecki, S. S., & McCarthy, P. J. (2010). Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy sediments in the Straits of Florida. The ISME Journal, 4(5), 686-699. doi:10.1038/ismej.2009.149
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1800.
Date Backup
2010
Date Text
2010
DOI
10.1038/ismej.2009.149
Date Issued (EDTF)
2010
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing13519", creator="creator:BCHANG", creation_date="2012-08-10 17:30:35", modified_by="super:FAUDIG", modification_date="2014-02-14 14:02:23"
IID
FADT3351982
Issuance
single unit
Person Preferred Name
Bruck, W. M.
creator
Physical Description
15 p.
Title Plain
Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy sediments in the Straits of Florida
Origin Information
Nature Publishing Group
2010
single unit
Title
Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy sediments in the Straits of Florida
Other Title Info
Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy sediments in the Straits of Florida