Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2023
EDTF Date Created
2023
Description
Epigenetic dysregulation has been implicated in oncogenesis, with post-translational histone modifications being linked to cancer progression. WSTF/BAZ1B forms chromatin-remodeling complexes with other proteins and lowers cancer survival outcomes. Treatment resistance causes >90 % of all cancer deaths. In particular, cancers develop tolerance to cisplatin-induced genotoxicity. It is hypothesized that the BAZ1B bromodomain, PHD finger, and DDT domain recognize epigenetic modifications, contributing to cisplatin resistance in cancers. To test this, the domains were expressed in Rosetta 2 BL21(DE3) and Rosetta 2 BL21(DE3) PLysS Escherichia coli strains. Soluble proteins were extracted, purified, and then analyzed using pulldown assays and modified histone peptide arrays. The DDT and PHD finger domains were found to bind to specific histone modifications with the DDT domain also displayed DNA-binding properties. Some of the identified histone modifications have known roles/correlations in normal and cancer cells, implicating BAZ1B as an agent in oncogenesis, treatment resistance, and as a therapeutic target.
Language
Type
Form
Extent
61 p.
Subject (Topical)
Identifier
FA00014296
Rights
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Additional Information
Includes bibliography.
Thesis (MS)--Florida Atlantic University, 2023.
FAU Electronic Theses and Dissertations Collection
Date Backup
2023
Date Created Backup
2023
Date Text
2023
Date Created (EDTF)
2023
Date Issued (EDTF)
2023
Extension
FAU
IID
FA00014296
Organizations
Attributed name: Florida Atlantic University
Attributed name: Department of Biomedical Science
Attributed name: Charles E. Schmidt College of Medicine
Person Preferred Name
Toussaint, Mohamed-Tamar
author
Graduate College
Physical Description
application/pdf
61 p.
Title Plain
CHARACTERIZATION OF BAZ1B, A TARGET PROTEIN OF CHEMOTHERAPEUTIC RESISTANCE IN CANCER
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Origin Information
2023
2023
Florida Atlantic University
Boca Raton, Fla.
Place
Boca Raton, Fla.
Title
CHARACTERIZATION OF BAZ1B, A TARGET PROTEIN OF CHEMOTHERAPEUTIC RESISTANCE IN CANCER
Other Title Info
CHARACTERIZATION OF BAZ1B, A TARGET PROTEIN OF CHEMOTHERAPEUTIC RESISTANCE IN CANCER