The purpose of this study was to determine the effect of cadence on time trial performance in novice female cyclists. Ten novice female cyclists volunteered to participate in this study. Participants performed 3 sessions: one VO2 peak and two time trials (TT). Cadence was randomly selected and fixed for each TT (60 or 100 rpm) while power output (PO) was adjusted by the participant, as tolerated. Finish time, HR, blood lactate, PO, VO2, and RPE were measured throughout the time trials. The major finding of this study was the significantly faster (p<0.05) finish time (minutes) during the 60 rpm condition (34:23) versus the 100 rpm condition (37:34). Also the 60 rpm TT resulted in significant (p<0.05) differences for HR (155.9 vs 161.2 bpm), gross efficiency (21.1% vs 17.7%), and PO (147 vs 129 watts). These results indicate that novice female cyclists benefit from adopting a low cadence during an 8k TT.