Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2007
EDTF Date Created
2007
Description
The Mexican axolotl (Ambystoma mexicanum) carries a cardiac lethal mutation
resulting in mutant embryos with no heartbeat. This homozygous recessive gene results
in tropomyosin deficiency and absence of organized myofibrils. Co-culturing mutant
hearts with bioactive RNA, termed myofibril-inducing RNA (MIR), from normal axolotl
embryonic anterior endoderm causes the mutant hearts to beat. It is hypothesized that the
secondary structure of the MIR binds a specific protein(s) and this is required to
synthesize tropomyosin and form organized myofibrils. In this study mutant hearts are
co-cultured with human fetal and adult heart total RNA to assess rescue of the mutant
hearts. Results show that both human fetal and adult heart total RNA rescue the mutant
condition in a manner similar to the MIR. Thus, the MIR human functional homologs
induce events leading to normal heart differentiation and function. This finding may help
people with heart muscle damage regain normal heart function again.
resulting in mutant embryos with no heartbeat. This homozygous recessive gene results
in tropomyosin deficiency and absence of organized myofibrils. Co-culturing mutant
hearts with bioactive RNA, termed myofibril-inducing RNA (MIR), from normal axolotl
embryonic anterior endoderm causes the mutant hearts to beat. It is hypothesized that the
secondary structure of the MIR binds a specific protein(s) and this is required to
synthesize tropomyosin and form organized myofibrils. In this study mutant hearts are
co-cultured with human fetal and adult heart total RNA to assess rescue of the mutant
hearts. Results show that both human fetal and adult heart total RNA rescue the mutant
condition in a manner similar to the MIR. Thus, the MIR human functional homologs
induce events leading to normal heart differentiation and function. This finding may help
people with heart muscle damage regain normal heart function again.
Language
Type
Form
Extent
49 p.
Subject (Topical)
Identifier
FA00000823
Additional Information
Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2007.
FAU Electronic Theses and Dissertations Collection
Charles E. Schmidt College of Medicine
Date Backup
2007
Date Created Backup
2007
Date Text
2007
Date Created (EDTF)
2007
Date Issued (EDTF)
2007
Extension
FAU
IID
FA00000823
Organizations
Attributed name: Florida Atlantic University
Attributed name: Charles E. Schmidt College of Medicine
Attributed name: Department of Biomedical Science
Person Preferred Name
Rueda-de-Leon, Elena
Graduate College
Physical Description
application/pdf
49 p.
Title Plain
Fetal and Adult Human Heart RNA Promotes Myofibrillogenesis and Stimulates a Heart Beat in Cardiac Non-function Mutant Mexican Axolotl Hearts in Organ Culture
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
2007
2007
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Fetal and Adult Human Heart RNA Promotes Myofibrillogenesis and Stimulates a Heart Beat in Cardiac Non-function Mutant Mexican Axolotl Hearts in Organ Culture
Other Title Info
Fetal and Adult Human Heart RNA Promotes Myofibrillogenesis and Stimulates a Heart Beat in Cardiac Non-function Mutant Mexican Axolotl Hearts in Organ Culture