Recovery of functional human lymphocytes from Leukotrap* filters

Member of
HBOI Faculty Contributions
Contributors
Longley, Ross E.
Stewart, D.
Publisher
Elsevier B.V.
Date Issued
1989
Note

We report here a method by which functional human PBL may be recovered from a blood product preparation unit (Leukotrap) which is normally employed by blood banks to prepare a leukocyte-poor blood product and which is commonly discarded after use. Blood filters which were back-flushed with cold, phosphate-buffered saline (PBS) and the resulting buffy coats collected and separated on Histopaque gradients yielded an average of 2.7 × 108 (±1.17 × 108) cells per filter processed (n = 38), with viability greater than 95%.

Language
English
Type
Text
Genre
article
Extent
7 p.
Subject (Topical)
Lymphocytes
Leucocytes
Blood--Filtration
Identifier
3353830
Additional Information
We report here a method by which functional human PBL may be recovered from a blood product preparation unit (Leukotrap) which is normally employed by blood banks to prepare a leukocyte-poor blood product and which is commonly discarded after use. Blood filters which were back-flushed with cold, phosphate-buffered saline (PBS) and the resulting buffy coats collected and separated on Histopaque gradients yielded an average of 2.7 × 108 (±1.17 × 108) cells per filter processed (n = 38), with viability greater than 95%.
The final published version of this manuscript is available at http://www.sciencedirect.com/science/journal/00221759 and may be cited as: Longley, R. E., & Stewart, D. (1989). Recovery of functional human lymphocytes from Leukotrap filters. Journal of Immunological Methods, 121(2), 33-38.doi:10.1016/0022-1759(89)90416-X
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #692.
Date Backup
1989
Date Text
1989
DOI
10.1016/0022-1759(89)90416-X
Date Issued (EDTF)
1989
Extension


FAU
FAU
admin_unit="FAU01", ingest_id="ing13945", creator="creator:BCHANG", creation_date="2012-10-29 12:23:19", modified_by="super:FAUDIG", modification_date="2014-02-06 14:21:41"

IID
FADT3353830
Issuance
single unit
Organizations
Attributed name: Harbor Branch Oceanographic Institute
Person Preferred Name

Longley, Ross E.

creator

Physical Description

pdf
7 p.
PURL
http://purl.flvc.org/FCLA/DT/3353830
Title Plain
Recovery of functional human lymphocytes from Leukotrap* filters
URL
http://purl.flvc.org/FCLA/DT/3353830
Origin Information

Elsevier B.V.
1989
single unit
Title
Recovery of functional human lymphocytes from Leukotrap* filters
Other Title Info

Recovery of functional human lymphocytes from Leukotrap* filters