Marine sponges are the source of many bioactive compounds with therapeutic potential. A critical issue
in the drug development strategy for marine natural products is ensuring an adequate supply of
compounds for clinical use while protecting the source organism and its habitat from over-exploitation.
One approach is the development of techniques for in vitro production of bioactive compounds.
Replicative cultures of the sponge Teichaxinella morchella have been established. Techniques for
monitoring sponge cell responses to growth factors by semi-automated microliter plate assays were
developed, including the sulforhodamine B method for total protein and a Hoechst 33342 DNA dye-binding
assay. The identity of cell cultures stimulated to divide by vertebrate growth factors and lectins was
verified by the in vitro production of stevensine (odiline), a compound which is ctiaracteristic of T.
morchella. Cultures retained their ability to synthesize stevensine after doubling, which demonstrates the
feasibility of in vitro production of bioactive, sponge-derived natural products.
Member of
Contributors
Publisher
Science Publishers
Date Issued
1997
Note
Language
Type
Genre
Form
Extent
8 p.
Subject (Topical)
Identifier
FA00007344
Additional Information
Marine sponges are the source of many bioactive compounds with therapeutic potential. A critical issue
in the drug development strategy for marine natural products is ensuring an adequate supply of
compounds for clinical use while protecting the source organism and its habitat from over-exploitation.
One approach is the development of techniques for in vitro production of bioactive compounds.
Replicative cultures of the sponge Teichaxinella morchella have been established. Techniques for
monitoring sponge cell responses to growth factors by semi-automated microliter plate assays were
developed, including the sulforhodamine B method for total protein and a Hoechst 33342 DNA dye-binding
assay. The identity of cell cultures stimulated to divide by vertebrate growth factors and lectins was
verified by the in vitro production of stevensine (odiline), a compound which is ctiaracteristic of T.
morchella. Cultures retained their ability to synthesize stevensine after doubling, which demonstrates the
feasibility of in vitro production of bioactive, sponge-derived natural products.
in the drug development strategy for marine natural products is ensuring an adequate supply of
compounds for clinical use while protecting the source organism and its habitat from over-exploitation.
One approach is the development of techniques for in vitro production of bioactive compounds.
Replicative cultures of the sponge Teichaxinella morchella have been established. Techniques for
monitoring sponge cell responses to growth factors by semi-automated microliter plate assays were
developed, including the sulforhodamine B method for total protein and a Hoechst 33342 DNA dye-binding
assay. The identity of cell cultures stimulated to divide by vertebrate growth factors and lectins was
verified by the in vitro production of stevensine (odiline), a compound which is ctiaracteristic of T.
morchella. Cultures retained their ability to synthesize stevensine after doubling, which demonstrates the
feasibility of in vitro production of bioactive, sponge-derived natural products.
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution 1172
This manuscript is an author version with the final publication
available and may be cited as: Pomponi, S. A., Willoughby, R., Kaighn, M. E., & Wright, A. E. (1997).
Development of techniques for in vitro production of bioactive natural products from marine sponges.
In K. Maramorosch & J. Mitsuhashi (Eds.), Invertebrate cell culture: novel directions and biotechnology
applications (pp. 231-237). Enfield, NH: Science Publishers.
available and may be cited as: Pomponi, S. A., Willoughby, R., Kaighn, M. E., & Wright, A. E. (1997).
Development of techniques for in vitro production of bioactive natural products from marine sponges.
In K. Maramorosch & J. Mitsuhashi (Eds.), Invertebrate cell culture: novel directions and biotechnology
applications (pp. 231-237). Enfield, NH: Science Publishers.
Date Backup
1997
Date Text
1997
Date Issued (EDTF)
1997
Extension
FAU
IID
FA00007344
Organizations
Attributed name: Kaighn, M. E.
Person Preferred Name
Pomponi, Shirley A.
spomponi@fau.edu
Physical Description
8 p.
Title Plain
Development of techniques for in vitro production of bioactive natural products from marine sponges
Origin Information
1997
Science Publishers
Enfield, NH
Place
Enfield, NH
Title
Development of techniques for in vitro production of bioactive natural products from marine sponges
Other Title Info
Development of techniques for in vitro production of bioactive natural products from marine sponges