A taxonomic survey was conducted to determine the microbial diversity held within the Harbor Branch Oceanographic Marine Microbial Culture Collection (HBMMCC). The collection consists of approximately 17,000 microbial isolates, with 11,000 from a depth of greater than 150 ft seawater. A total of 2273 heterotrophic bacterial isolates were inventoried using the DNA fingerprinting technique amplified rDNA restriction analysis on approximately 750–800 base pairs (bp) encompassing hypervariable regions in the 50 portion of the small subunit (SSU) 16S rRNA gene.
This is the author’s version of a work accepted for publicationby Elsevier. Changes resulting from the publishing process, including peer review, editing,corrections, structural formatting and other quality control mechanisms, may not be reflectedin this document. Changes may have been made to this work since it was submitted forpublication. The definitive version has been published at http://www.elsevier.de/syapm andmay be cited as Sfanos, Karen, Dedra Harmody, Phat Dang, Angela Ledger, Shirley Pomponi,Peter McCarthy, Jose Lopez (2004) A molecular systematic survey of cultured microbialassociates of deep‐water marine invertebrates, Systematic and Applied Microbiology 28:242–264 doi:10.1016/j.syapm.2004.12.002
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1569.