Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University Digital Library
Date Issued
2011
EDTF Date Created
2011
Description
Reactive oxygen species (ROS) are molecular oxygen-derived molecules
that are exceedingly reactive, often generated as free radical bi-products of
mitochondrial respiration, which cause oxidative stress that leads to aging. To
avoid the generation of ROS, aerobic organisms have antioxidant defense
mechanisms that use enzymes such as Superoxide Dismutase (SOD) to convert
the superoxide radicals into hydrogen peroxide and oxygen. Manganesecontaining
SOD (Mn-SOD), a product of the SOD2 gene is found in all eukaryotic
organisms in the mitochondrial matrix, including T. scripta (red-eared slider
turtle). Extraordinarily, T. scripta can live without oxygen for long periods of
time without experiencing adverse effects when oxygen is again available. The
purpose of our experiment is to clone the T. scripta SOD2 gene, and test its
activity. A partial fragment of the gene has been isolated by screening the cDNA
library, and we are currently working on finding the remaining sequence.
that are exceedingly reactive, often generated as free radical bi-products of
mitochondrial respiration, which cause oxidative stress that leads to aging. To
avoid the generation of ROS, aerobic organisms have antioxidant defense
mechanisms that use enzymes such as Superoxide Dismutase (SOD) to convert
the superoxide radicals into hydrogen peroxide and oxygen. Manganesecontaining
SOD (Mn-SOD), a product of the SOD2 gene is found in all eukaryotic
organisms in the mitochondrial matrix, including T. scripta (red-eared slider
turtle). Extraordinarily, T. scripta can live without oxygen for long periods of
time without experiencing adverse effects when oxygen is again available. The
purpose of our experiment is to clone the T. scripta SOD2 gene, and test its
activity. A partial fragment of the gene has been isolated by screening the cDNA
library, and we are currently working on finding the remaining sequence.
Language
Type
Genre
Form
Extent
31 p.
Identifier
FA00003605
Rights
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Additional Information
Includes bibliography.
Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2011.
Florida Atlantic University Digital Library Collections
Date Backup
2011
Date Created Backup
2011
Date Text
2011
Date Created (EDTF)
2011
Date Issued (EDTF)
2011
Extension
FAU
IID
FA00003605
Organizations
Attributed name: Harriet L. Wilkes Honors College
Attributed name: Florida Atlantic University
Person Preferred Name
Sanchez, Jairo
author
Harriet L. Wilkes Honors College
Physical Description
online resource
31 p.
Title Plain
Isolating the Manganese Superoxide Dismutase Gene (SOD2) from T. scripta""
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
2011
2011
Florida Atlantic University Digital Library
Boca Raton, Fla.
Place
Boca Raton, Fla.
Title
Isolating the Manganese Superoxide Dismutase Gene (SOD2) from T. scripta""
Other Title Info
Isolating the Manganese Superoxide Dismutase Gene (SOD2) from T. scripta""