Targeted Gene Knock-out Via Promoter Tagging in Tomato (Lycopersicon Esculentum) and Tobacco (Nicotiana Tabacum).

Many different ways to create mutants have been established. This research
demonstrates yet another variation of the promoter tagging technique that allows for a
single step selection of the putative transgenic plants that have a mutation in
constitutively expressed genes. While tomato transformants have not yet been
convincingly confirmed, tobacco transformation resulted in seven transgenic lines
showing resistance to high concentrations of kanamycin. Two transgenic lines were
further investigated and three putative promoters isolated. Transient expression analysis
of leaves transformed by particle bombardment with vectors carrying beta-glucuronidase
gene driven by these putative promoters suggests two of them to be functional. Further
investigation is needed to confirm the expression in the stably transformed plants as well
as cloning of the genes downstream of the functional promoters and research of their
functions.