Note
FAU Electronic Theses and Dissertations Collection
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
1992
Description
Monoclonal antibodies (MAbs) were produced against sailfish (Istiophorus platypterus) albumin and investigated for their potential use in species identification. Balb/c mice were immunized with albumin purified from sailfish serum. Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) for reactivity with the purified albumins of sailfish, blue marlin (Makaira nigricans) and white marlin (Tetrapturus albidus). MAbs from 18 different hybridomas exhibited activity against sailfish albumin. Sixteen MAbs showed cross-reactivity with the marlin species. Two MAbs displayed distinct specificity for sailfish albumin. One of these MAbs (M2D1) was conjugated to horseradish peroxidase to construct an ELISA for the identification of sailfish from serum. The assay correctly identified 18 sailfish from 36 billfish samples. The MAb-peroxidase conjugate was highly specific toward sailfish with no detectable reaction against the heterologous species.
Language
Type
Form
Extent
58 p.
Subject (Topical)
Identifier
14801
Additional Information
FAU Electronic Theses and Dissertations Collection
Thesis (M.S.)--Florida Atlantic University, 1992.
Charles E. Schmidt College of Science
Date Backup
1992
Date Text
1992
Date Issued (EDTF)
1992
Extension
FAU
FAU
admin_unit="FAU01", ingest_id="ing1508", creator="staff:fcllz", creation_date="2007-07-19 03:10:58", modified_by="staff:fcllz", modification_date="2011-01-06 13:09:14"
IID
FADT14801
Issuance
monographic
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
Rossi, Edmund Anthony.
Graduate College
Physical Description
58 p.
application/pdf
Title Plain
Evaluation of the specificity and sensitivity of monoclonal antibodies produced against sailfish albumin
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
http://rightsstatements.org/vocab/InC/1.0/
Origin Information
1992
monographic
Boca Raton, Fla.
Florida Atlantic University
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Evaluation of the specificity and sensitivity of monoclonal antibodies produced against sailfish albumin
Other Title Info
Evaluation of the specificity and sensitivity of monoclonal antibodies produced against sailfish albumin