Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2013
EDTF Date Created
2013
Description
Aconitase is a multifunctional enzyme essential for mitochondrial DNA maintenance and cellular respiration. For my thesis, I used an error prone PCR mutagenesis method to mutate the yeast aconitase (ACO1) gene and created a mutant library of Sacchromyces cerevisiae yeast cells. The library underwent 50oC heat shocks to select for cells expressing an aconitase mutant that increased the cells’ resistance to heat shock-induced oxidative stress. Oxidative stress has been implicated as a theory of cellular aging. Isolated aconitase mutants with increased oxidative stress resistance will be assayed for increased lifespan, which would support the free radical theory of aging and implicate aconitase as an important determinant of cellular aging.
Language
Type
Genre
Form
Extent
26 p.
Identifier
FA00003511
Rights
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Additional Information
Includes bibliography.
Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2013.
Date Backup
2013
Date Created Backup
2013
Date Text
2013
Date Created (EDTF)
2013
Date Issued (EDTF)
2013
Extension
FAU
IID
FA00003511
Organizations
Attributed name: Harriet L. Wilkes Honors College
Person Preferred Name
Enogieru, Imarhia
Physical Description
26 p.
born digital
Title Plain
Some like it hot: the isolation of an aconitase mutant resistant to heat shock-induced oxidative stress
Use and Reproduction
Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Digital Origin
born digital
Origin Information
2013
2013
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
FAU Digital Library
Title
Some like it hot: the isolation of an aconitase mutant resistant to heat shock-induced oxidative stress
Other Title Info
Some like it hot: the isolation of an aconitase mutant resistant to heat shock-induced oxidative stress