Note
Thesis (M.S.)--Florida Atlantic University, 1967.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
1967
EDTF Date Created
1967
Description
The disease of equine infectious anemia
(EIA), since the first records in 1847, has been one
of the most serious and deadly virus diseases of the
Equidae. The most difficult factor concerning this
disease was adequate diagnosis. The clinical diagnosis
has proven itself undependable and until 1966 with the
Immune-adherence test the laboratory diagnosis was
inconclusive. The indirect passive hemagglutination
test was used as a possible test for the disease due
to its high sensitivity. The indirect passive hemagglutination as
the diagnostic test for EIA was based on the use of
antibody as the titrating agent instead of the virus.
The antibody was removed from the sera of infected
horses. The seperation of the antibody was
accomplished by use of a DEAE-cellulose column and an
increasing salt gradient. The various proteins were
sepRrated and measured in a spectrophotometer. The
antibody portion was collected and concentrated by
polyethylene gycol. The more specific type of antibody
(19S) was used in all tests. The indirect passive hemagglutination test
was run on a total of 92 sera of which 58 were
negative and 34 sera were positive. The test proved
reliable in all the sera tested. In support of the
IPHA the IA test was run as a comparison.
(EIA), since the first records in 1847, has been one
of the most serious and deadly virus diseases of the
Equidae. The most difficult factor concerning this
disease was adequate diagnosis. The clinical diagnosis
has proven itself undependable and until 1966 with the
Immune-adherence test the laboratory diagnosis was
inconclusive. The indirect passive hemagglutination
test was used as a possible test for the disease due
to its high sensitivity. The indirect passive hemagglutination as
the diagnostic test for EIA was based on the use of
antibody as the titrating agent instead of the virus.
The antibody was removed from the sera of infected
horses. The seperation of the antibody was
accomplished by use of a DEAE-cellulose column and an
increasing salt gradient. The various proteins were
sepRrated and measured in a spectrophotometer. The
antibody portion was collected and concentrated by
polyethylene gycol. The more specific type of antibody
(19S) was used in all tests. The indirect passive hemagglutination test
was run on a total of 92 sera of which 58 were
negative and 34 sera were positive. The test proved
reliable in all the sera tested. In support of the
IPHA the IA test was run as a comparison.
Language
Type
Form
Extent
55 p.
Subject (Topical)
Identifier
FA00000758
Additional Information
Thesis (M.S.)--Florida Atlantic University, 1967.
Date Backup
1967
Date Created Backup
1967
Date Text
1967
Date Created (EDTF)
1967
Date Issued (EDTF)
1967
Extension
FAU
IID
FA00000758
Organizations
Attributed name: Florida Atlantic University
Person Preferred Name
Flynn, John Howard
author
Graduate College
Physical Description
application/pdf
55 p.
Title Plain
Indirect Passive Hemagglutination as a Diagnostic Test for Equine Infectious Anemia
Use and Reproduction
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Origin Information
1967
1967
Florida Atlantic University
Boca Raton, Fla.
Physical Location
Florida Atlantic University Libraries
Place
Boca Raton, Fla.
Sub Location
Digital Library
Title
Indirect Passive Hemagglutination as a Diagnostic Test for Equine Infectious Anemia
Other Title Info
Indirect Passive Hemagglutination as a Diagnostic Test for Equine Infectious Anemia