Note
Includes bibliography.
Member of
Contributors
Publisher
Florida Atlantic University
Date Issued
2018
EDTF Date Created
2018
Description
Parkinson’s Disease (PD) is a neurodegenerative disorder that affects millions of
people around the world, although it is more common in individuals aged 60 years or
older. PD is associated with the degeneration of dopamine neurons in the substantia
nigra. While the underlying cause of neuronal degeneration is poorly understood,
mitochondrial dysfunction is a common feature of the cell pathology. Geneticallyencoded
fluorescent probes were used to label the mitochondria in wildtype Drosophila
melanogaster and those genetically manipulated to model PD. Brains were dissected,
immunolabeled, and their mitochondria were imaged using structured illumination
microscopy (SIM). Mitochondrial number was determined, as well as the sphericity and
surface area quantified. This characterization of mitochondrial number and morphology
in wildtype Drosophila created a baseline for comparison to Drosophila that over-express
the wildtype human α-synuclein protein which is associated with PD.
people around the world, although it is more common in individuals aged 60 years or
older. PD is associated with the degeneration of dopamine neurons in the substantia
nigra. While the underlying cause of neuronal degeneration is poorly understood,
mitochondrial dysfunction is a common feature of the cell pathology. Geneticallyencoded
fluorescent probes were used to label the mitochondria in wildtype Drosophila
melanogaster and those genetically manipulated to model PD. Brains were dissected,
immunolabeled, and their mitochondria were imaged using structured illumination
microscopy (SIM). Mitochondrial number was determined, as well as the sphericity and
surface area quantified. This characterization of mitochondrial number and morphology
in wildtype Drosophila created a baseline for comparison to Drosophila that over-express
the wildtype human α-synuclein protein which is associated with PD.
Language
Type
Genre
Form
Extent
51 p.
Identifier
FAUHT00032
Additional Information
Includes bibliography.
Thesis (B.A.)--Florida Atlantic University, Harriet L. Wilkes Honors College, 2018.
FAU Honors Theses Digital Collection
Date Backup
2018
Date Created Backup
2018
Date Text
2018
Date Created (EDTF)
2018
Date Issued (EDTF)
2018
Extension
FAU
IID
FAUHT00032
Organizations
Attributed name: Florida Atlantic University
Attributed name: Harriet L. Wilkes Honors College
Person Preferred Name
Murphy, Regina
author
Physical Description
application/pdf
51 p.
Title Plain
MAPPING MITOCHONDRIAL NUMBER AND MORPHOLOGY IN THE BRAINS OF DROSOPHILA MELANOGASTER MODELS OF PARKINSON’S DISEASE
Use and Reproduction
Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Origin Information
2018
2018
Florida Atlantic University
Jupiter, Florida
Physical Location
Florida Atlantic University Libraries
Place
Jupiter, Florida
Sub Location
Digital Library
Title
MAPPING MITOCHONDRIAL NUMBER AND MORPHOLOGY IN THE BRAINS OF DROSOPHILA MELANOGASTER MODELS OF PARKINSON’S DISEASE
Other Title Info
MAPPING MITOCHONDRIAL NUMBER AND MORPHOLOGY IN THE BRAINS OF DROSOPHILA MELANOGASTER MODELS OF PARKINSON’S DISEASE