The effect of acclimation time, habituation period, and final freshwater ion composition on the survival of freshwater-acclimated Pacific white shrimp Litopenaeus vannamei postlarvae was investigated. During each of three experiments, shrimp were acclimated from 30 ppt to freshwater ( < 1 ppt TDS) utilizing various acclimation times (32, 40, 48, 72 h) with a constant or variable rate of salinity reduction. Shrimp were then held at the final acclimation salinity for 0, 1 or 2 days (habituation period) before being transferred to challenge ion treatment solutions. Ion treatment solutions derived from chloride-based chemicals were of the same total ion concentration, but either strongly monovalent or strongly divalent.
This is the author’s version of a work accepted for publication by Elsevier. Changes resulting from the publishing process, including peer review, editing, corrections, structural formatting and other quality control mechanisms, may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. The definitive version has been published at http://www.elsevier.com/locate/aqua‐online and may be cited as McGraw, William J., John Scarpa (2004) Mortality of freshwater-acclimated Litopenaeus vannamei associated with acclimation rate, habituation period, and ionic challenge, Aquaculture 236(1-4):285–296 doi:10.1016/j.aquaculture.2004.01.037
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution #1527.