Space perception

Spatial-based attention is shown to vary in strength over short intervals of time. Whether object-based selection also has similar temporal variability is not known. Egly, Driver and Rafal (1994) demonstrated using 2-rectangle displays how both spatial and object-based selection engages in processing of a visual scene. In Experiment-1 using the 2-rectangle paradigm we measured temporal variability of target detection by presenting targets at a variable SOA. In Experiment-2, we used 4-squares to preclude any object-based selection and measured temporal variability in target detection at similar locations as in Experiment-1. We found target detection to be periodic in delta and theta hertz rhythm in both Experiment-1 and Experiment-2 upon comparing corresponding cue-valid and same-object locations. Similar spectral profiles across experiments indicate a split-spotlight of spatial attention that rhythmically monitors cue-valid and other invalid locations. Future experiments are needed to determine whether object-based selection is periodic in nature.

The hippocampal-medial prefrontal circuit has been shown to serve a critical role
in decision making and goal directed actions. While the hippocampus (HF) exerts a direct
influence on the medial prefrontal cortex (mPFC), there are no direct return projections
from the mPFC to the HF. The nucleus reuniens (RE) of the midline thalamus is strongly
reciprocally connected with the HF and mPFC and represents the major link between
these structures.
We investigated the role of RE in functions associated with the hippocampus and
the mPFC -- or their interactions. Using two different inactivation techniques
(pharmacological and chemogenetic), we sought to further define the role of RE in spatial
working memory (SWM) and behavioral flexibility using a modified delayed non-match
to sample (DNMS) working memory task. We found that the reversible inactivation of
RE with muscimol critically impaired SWM performance, abolished well-established
spatial strategies and produced a profound inability to correct non-rewarded, incorrect choices on the T-maze (perseverative responding). We observed similar impairments in
SWM following the chemogenetic (DREADDs) inactivation of RE or selective RE
projections to the ventral HF. In addition, we showed that the inhibition of RE terminals
to the dorsal or ventral HF altered task related behaviors by increasing or decreasing the
time to initiate the task or reach the reward, respectively. Finally, we examined discharge
properties of RE cells across sleep-wake states in behaving rats. We found that the
majority of RE cells discharge at high rates of activity in waking and REM and at
significantly reduced rates in SWS, with a subpopulation firing rhythmically in bursts
during SWS. We identified five distinct subtypes of RE cells that discharged differently
across vigilant states; those firing at highest rates in waking (W1, W2), in REM sleep
(R1, R2) and SWS (S1). Given the differential patterns of activity of these cells, we
proposed they may serve distinct functions in waking – and possibly in SWS/REM sleep.
In sum, our findings indicate that RE is critically involved in mnemonic and
executive functions and the heterogeneous activity of these cells support a role for RE in
arousal/attention, spatial working memory and cognition.

The effects of the onset, offset, and sustained presence of inducing lines on the perceived position of test lines were independently investigated in a vernier alignment task. For spatial separations larger than 2.3 min, repulsion effects were always observed. For the smallest spatial separation, 2.3 min, the effect of the inducing lines was attraction for 0 and 195 SOAs. Minimal attraction was observed for a 3000 SOA. However, when the offset effect was isolated using the 3000 SOA and a 0 ISI, a large repulsion effect was observed for the 2.3 min spatial separation, as well as for the larger spatial separations. These results indicate that the temporal separation between visual elements is as important in determining perceived position as their spatial separation, which has been demonstrated in earlier studies (Badcock & Westheimer, 1985). A differential gradient model is proposed which accounts for these findings in terms of excitatory and inhibitory interactions within an ensemble of position-sensitive units.

When perceivers examine a visual scene, they can control the extent to which their attention is either narrowly focused or spread over a larger spatial area. The experiments reported in this dissertation explore the consequences of narrow vs. broad attention for simple spatial discriminations as well as more complex cooperative interactions that are the basis for the self-organization of coherent motion patterns. Subjects' attentional spread (narrow or broad) is manipulated by means of a primary, luminance detection task. In conjunction with the luminance detection task is a secondary, spatial discrimination or detection task, which differs in the four reported experiments. In Experiment 1, the discrimination of misalignment of two visual elements is enhanced by narrowly focused attention. In Experiment 2, discrimination of horizontal spatial separation of two visual elements is improved for small inter-element distances by narrow attention and for relatively large inter-element distances by broad attention. Experiment 3 shows that the inter-element distance among counterphase-presented visual elements for which unidirectional and oscillatory motion patterns are observed with equal frequency depends on subjects' attentional spread. Narrow attention favors the oscillatory pattern and broad attention favors the unidirectional pattern. Experiment 4 shows that attentional spread has a minimal effect on the detection of motion, and, additionally that attentional effects on simple spatial judgments (Experiments 1 and 2) are too small to account for the large shift in the equi-probable boundary of reported unidirectional and oscillatory motion patterns found in Experiment 3. Therefore, it is concluded in conjunction with Hock and Balz's (1994) differential gradient model, that attentional spread influences the self-organization of unidirectional and oscillatory motion patterns through its effects on the relative strength of facilitating and inhibiting interactions among directionally selective motion detectors.

Male C7BL/6J mice were implanted with bilateral dorsal CA1 guide cannulae. After confirming that intrahippocampal microinfusion of muscimol impaired hippocampal function, demonstrated by impaired performance in the Morris water maze, the influence of intrahippocampal muscimol was tested in the Novel Object Recognition paradigm. During a test session 24 h after the last habituation/sample session, mice were presented with one familiar object and one novel object. Successful retention of object memory was inferred if mice spent more time exploring the novel object than the familiar object. Results demonstrate that muscimol infused into dorsal CA1 region prior to the test session eliminates novel object preference, indicating that the hippocampus is necessary for the retrieval of this non-spatial memory - a topic that has garnered much debate. Understanding the similarities between rodent and human hippocampal function could enable future animal studies to effectively answer questions about diseases and disorders affecting human learning and memory.