Estuarine ecology

Eutrophication of urbanized estuaries is a global issue that continues to worsen as coastal development increases. The Indian River Lagoon (IRL) on Florida’s east-central coast is a eutrophic estuary that is experiencing harmful algal blooms of macroalgae and phytoplankton, as well as widespread seagrass losses. This is concerning as seagrasses provide many ecosystem services, including the provision of essential habitat. These alterations in benthic cover can have ecosystem level effects and require further investigation. Thus, drivers and effects of primary producer alterations in the IRL were investigated through analysis of long-term monitoring data, field surveys of faunal densities inhabiting macroalgae and bare bottom habitats, and stable nitrogen isotope (δ15N) analyses of primary producers, primary consumers, and secondary consumers. Long-term monitoring data from the northern IRL (NIRL) and Banana River Lagoon (BR) demonstrated there have been major seagrass losses coupled with increases in occurrence of the rhizophytic green macroalgae Caulerpa prolifera, which is now the dominant benthic cover in many locations. Multivariate analyses of long-term monitoring data spanning 2011-2020 suggested that the carbon to phosphorus ratio (C:P) of macroalgae is an important factor related to annual changes in benthic cover in the NIRL and BR; increased P-availability is correlated with these primary producer shifts. In situ collections of macroinvertebrates and resident fishes showed the current function and importance of macroalgae as habitat in the NIRL and BR, particularly in the relative absence of seagrass.

Estuarine ecosystems are dynamic habitats, where the convergence of marine and freshwater results in constant fluxes in environmental abiotic parameters. Organisms must balance these variations within their optimal range to minimize physiological costs, often by movement from unsuitable to more suitable areas. Additional disruptions to ecosystem balances, such as anthropogenic hydrologic discharges, further alter environmental conditions and may cause population-wide movement responses of mobile organisms. Responses to anthropogenic and natural fluctuations can differ based on time of year, life history stage, or individual characteristics. These ecologically-balanced dynamics are difficult to model. In this study, I examined variability in estuarine environmental data and common snook (Centropomus undecimalis) movement responses to anthropogenic and natural fluctuations in the environment in a managed waterway. ARIMA time series models were tested as a method of modeling variability in environmental parameters. Monthly variance was well described throughout most of the estuary, especially when the interannual and intra-annual patterns were stable, indicating that these models are a good method for these types of data and could be appropriate for forecasting. Euryhaline sportfish movement responses to high discharge events in a managed waterway were observed with passive acoustic telemetry and did not show large-scale, population-wide consistency. Responses were variable between and within individuals, but individual characteristics appear to have influenced behavior in response to disturbances. Thus, these sportfish populations may be more resilient to this type of disturbance than previously hypothesized. Generalized additive mixed effects models showed that the distribution and movement of individual fishes varied in response to multiple natural and anthropogenic factors, and there was no primary driver. The understanding of the relationships among the distribution and movement of fishes and abiotic and anthropogenic factors can guide management of waterways and provide insight into how changes will affect abiotic factors and communities.

The Indian River Lagoon (IRL) FL, USA, is an Estuary of National Significance due to its economic and high biodiversity. Microbial populations are understudied in the IRL despite their numerous ecological services. A two-year, nineteen-site Lagoon-Wide Survey (LWS) was conducted to provide the first 16S rRNA amplicon sequencing data on the microbiome of the sediment in the IRL and determine how the microbiome changed in response to environmental and anthropogenic factors. The most influential variables that explained the variability between microbiomes were porewater salinity, total organic matter (TOM), and copper (Cu). These results correlated with some of the anthropogenic pressures the IRL faces such as freshwater discharges from St. Lucie Estuary (SLE), trace metal contamination, and the accumulation of fine-grained, highly organic sediment known as “IRL muck” (muck). Research then focused on determining the microbial differences between three sets of sample types: sediment from the IRL versus the SLE; sediment that had three muck characteristics versus those with zero; and high TOM sediment that had high Cu versus high TOM sediment that had low Cu. Differentially abundant prokaryotes between sample types were determined with novel indicator analysis techniques. One technique tested the effectiveness of an indicator list to separate samples based upon the product of the sensitivity and specificity of partitioning around medoids clustering in comparison to metadata classifications. The other technique allowed for the tracking of changes in the entire indicator microbiome. These new indicator analysis techniques were created using the original LWS data and tested to determine how sediment microbiomes responded during two opportunistic surveys: dredging of muck from an IRL tributary (Eau Gallie River) and Hurricane Irma. These studies have filled the knowledge gap regarding the unknown microbiome of the IRL and how sediment microbiomes respond to extreme events such as dredging and a hurricane. They also led to the development of new indicator analysis techniques that can be used by to track changes in the entire indicator microbiome.

A brown tide bloom of the pelagophyte Aureoumbra lagunensis caused significant impacts to north Indian River Lagoon (IRL) in 2012-2013, including seagrass die-offs, fish kills, and reduced growth and grazing of ecologically important bivalves. There is potential for another pelagophyte, Aureococcus anophagefferens, to expand into this system. Filtration rates (FR) of the pleated tunicate Styela plicata exposed to Aureoumbra lagunensis and Aureococcus anophagefferens were measured against exposure to a control alga (Tisochrysis lutea) in order to determine its potential use as a bioremediator against these harmful algal blooms (HABs). In addition, whether S. plicata might serve as a vector of HABs was studied by culturing fecal deposits. Short-term exposure to HABs significantly reduced FR, whereas long-term exposure indicates comparable cell removal compared to the control. Vector potential of S. plicata was inconclusive. Results warrant further research to determine whether S. plicata can acclimate or respond to HAB conditions over time.

When onsite treatment and disposal systems (OSTDS) are not properly sited, they can be a potential risk to public health and a source of environmental degradation. In Florida, OSTDS location has proven to be problematic from a water resources perspective. The objective of this study is to quantify the pollutant loading contributions from OSTDS on coastal canals, with regard to nutrients and pathogens indicators (Total Coliform, E. coli, Enterococcus). Two similar single-family residential locations were identified: Dania Beach, FL (with 100% OSTDS) and Hollywood, FL (with 100% sewer). Field studies were conducted during the height of the wet and dry seasons. During the wet season, surface water quality of the canal was affected by an OSTDS contribution with higher levels of nutrients and pathogens indicators. During the dry season, no OSTDS impact was detected. OSTDS appear to work properly during the dry season.

This project tested the effects of water conditions on developmental rates of larvae of the estuarine sea anemone Nematostella vectensis . Egg masses were collected immediately following fertilization and placed in solutions with different salinities and maintained in a temperature-controlled water bath. Every twelve hours, embryonic development was checked for progression through different morphological stages of development. Comparison of regression analyses of larval development at each temperature increment indicated that both temperature and salinity affected the rate of development; development was slower in lower conditions and faster at higher salinities and temperatures, with extreme conditions inducing deleterious effects. The suggested ideal conditions for these larvae are a salinity range of 12.5--20% and a temperature range of 22--24C. These parameters provide a foundation from which a standardized testing method may be established, using N. vectensis larval development.